|
Status |
Public on Jul 13, 2022 |
Title |
08_MC38 APCKO_2 |
Sample type |
RNA |
|
|
Source name |
Tumors established with ishTDO2 APC-KO MC38 cells
|
Organism |
Mus musculus |
Characteristics |
cell line: MC38 genotype: APC-KO treatment: no Dox
|
Treatment protocol |
Inducible shTDO2 MC38 cell lines were injected to the C57BL/6 mouse cecum orthotopically and treated with Doxycycline water to induce TDO2 knockdown.
|
Growth protocol |
The CRC cell lines MC38 (RRID:CVCL_B288) and its isogenic cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% Tet System Approved FBS (Clontech) and 100 U/ml ampicillin/penicillin. All cells were confirmed to be mycoplasma-free and maintained at 37 °C and 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNAs were isolated using Trizol (Invitrogen, Cat# 15596-026) and further purified with the RNeasy Mini Kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
|
|
Hybridization protocol |
Biotinylated cRNA was fragmented and hybridized to Mouse Genome 430 2.0 Array following the standard protocols
|
Scan protocol |
Slides were scanned by GeneChip® Scanner
|
Description |
Tumors established with ishTDO2 APC-KO MC38 cells no Dox replicate 2
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings
|
|
|
Submission date |
Apr 25, 2022 |
Last update date |
Jul 13, 2022 |
Contact name |
Chang-Jiun Wu |
Organization name |
University of Texas, MD Anderson Cancer Center
|
Department |
Genomic Medicine
|
Lab |
DePinho Lab
|
Street address |
1515 Holcombe Blvd
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE200910 |
Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer |
GSE201415 |
Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer [array] |
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