|
| Status |
Public on Jul 13, 2022 |
| Title |
12_MC38 APCKO_3 Dox |
| Sample type |
RNA |
| |
|
| Source name |
Tumors established with ishTDO2 APC-KO MC38 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: MC38
genotype: APC-KO
treatment: Dox
|
| Treatment protocol |
Inducible shTDO2 MC38 cell lines were injected to the C57BL/6 mouse cecum orthotopically and treated with Doxycycline water to induce TDO2 knockdown.
|
| Growth protocol |
The CRC cell lines MC38 (RRID:CVCL_B288) and its isogenic cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% Tet System Approved FBS (Clontech) and 100 U/ml ampicillin/penicillin. All cells were confirmed to be mycoplasma-free and maintained at 37 °C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were isolated using Trizol (Invitrogen, Cat# 15596-026) and further purified with the RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was fragmented and hybridized to Mouse Genome 430 2.0 Array following the standard protocols
|
| Scan protocol |
Slides were scanned by GeneChip® Scanner
|
| Description |
Tumors established with ishTDO2 APC-KO MC38 cells plus Dox replicate 3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings
|
| |
|
| Submission date |
Apr 25, 2022 |
| Last update date |
Jul 13, 2022 |
| Contact name |
Chang-Jiun Wu |
| Organization name |
University of Texas, MD Anderson Cancer Center
|
| Department |
Genomic Medicine
|
| Lab |
DePinho Lab
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE200910 |
Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer |
| GSE201415 |
Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer [array] |
|
