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Sample GSM6069205 Query DataSets for GSM6069205
Status Public on Sep 06, 2023
Title R295_CD4
Sample type SRA
 
Source name Primary activated CD4 cells from PBMC
Organism Homo sapiens
Characteristics cell type: activated_CD4
agent: anti-CD3
disease: Rheumatoid arthritis
Sex: female
Treatment protocol culture activated with anti-CD3, 2h total
Growth protocol Lymphoprep separation of PBMC, Magnetic separation of CD4 cells,
Extracted molecule total RNA
Extraction protocol Total RNA purification (Norgen, Ontario, Canada)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description R295
mapped to HG38
Data processing Aligned: STAR, Ref.genome: Homo_sapiens.GRCh38.dna.primary_assembly.fa (Ensembl),
quantification: featureCounts
Assembly: HG38
Supplementary files format and content: matrix with ENS number and samples in each one column
 
Submission date Apr 27, 2022
Last update date Sep 06, 2023
Contact name Maria I Bokarewa
E-mail(s) maria.bokarewa@rheuma.gu.se
Organization name Gothenburg University
Department Rheumatology and Inflammation Research
Lab Bokarewa
Street address Guldhedagatan 10A
City Gothenburg
State/province VG
ZIP/Postal code SE41346
Country Sweden
 
Platform ID GPL11154
Series (1)
GSE201669 RNAseq of CD4 T cells from female Rheumatoid arthritis patients [NeumRA_CD4]
Relations
BioSample SAMN27868339
SRA SRX15014137

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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