|
Status |
Public on Sep 06, 2023 |
Title |
R295_CD4 |
Sample type |
SRA |
|
|
Source name |
Primary activated CD4 cells from PBMC
|
Organism |
Homo sapiens |
Characteristics |
cell type: activated_CD4 agent: anti-CD3 disease: Rheumatoid arthritis Sex: female
|
Treatment protocol |
culture activated with anti-CD3, 2h total
|
Growth protocol |
Lymphoprep separation of PBMC, Magnetic separation of CD4 cells,
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA purification (Norgen, Ontario, Canada)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
R295 mapped to HG38
|
Data processing |
Aligned: STAR, Ref.genome: Homo_sapiens.GRCh38.dna.primary_assembly.fa (Ensembl), quantification: featureCounts Assembly: HG38 Supplementary files format and content: matrix with ENS number and samples in each one column
|
|
|
Submission date |
Apr 27, 2022 |
Last update date |
Sep 06, 2023 |
Contact name |
Maria I Bokarewa |
E-mail(s) |
maria.bokarewa@rheuma.gu.se
|
Organization name |
Gothenburg University
|
Department |
Rheumatology and Inflammation Research
|
Lab |
Bokarewa
|
Street address |
Guldhedagatan 10A
|
City |
Gothenburg |
State/province |
VG |
ZIP/Postal code |
SE41346 |
Country |
Sweden |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE201669 |
RNAseq of CD4 T cells from female Rheumatoid arthritis patients [NeumRA_CD4] |
|
Relations |
BioSample |
SAMN27868339 |
SRA |
SRX15014137 |