|
| Status |
Public on Jun 12, 2005 |
| Title |
TriMeH3K27 in HeLa cell - rep2 of 3 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Chromatin immunoprecipiated DNA using trimethyl-K27 histone H3 antibody, HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
Human cervix epithelial adenocarcinoma cells, HeLa cells, trimethyl-K27 histone H3 antibody
|
| Extracted molecule |
genomic DNA |
| Label |
Cy5
|
| |
|
| Channel 2 |
| Source name |
Input DNA from HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
Human cervix epithelial adenocarcinoma cells, HeLa cells
|
| Extracted molecule |
genomic DNA |
| Label |
Cy3
|
| |
|
| |
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 4.0 analysis software.
|
| Description |
Genome-wide location analysis of TriMeH3K27 binding in HeLa cells using RenLab ENCODE PCR tiling array. Array ID is ENC853. Replicate 2 of 3.
|
| Data processing |
The two channels in each array are normalized using printtip loess with normexp background correction using R. The replicate arrays are then quantile normalized using reference channel intensity. The normalized intensities are then used for p-value calculation using single array error model, and log2 ratio calculation.
|
| |
|
| Submission date |
Jun 09, 2005 |
| Last update date |
Jun 10, 2005 |
| Contact name |
Chunxu Qu |
| E-mail(s) |
qchunxu@ucsd.edu
|
| Phone |
(858)822-5767
|
| Organization name |
Ludwig Institute for Cancer Research
|
| Department |
Gene Regulation
|
| Lab |
RenLab
|
| Street address |
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL1454 |
| Series (1) |
| GSE2801 |
ENCODE: GWLA analysis of SUZ12 and triMeH3K27 binding in HeLa cells |
|
