|
| Status |
Public on Oct 15, 2010 |
| Title |
HC_77184 |
| Sample type |
RNA |
| |
|
| Source name |
blood PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: PBMCs
patient status: healthy control (HC)
ana status: ANA+
sample number: 77184
|
| Extracted molecule |
total RNA |
| Extraction protocol |
2ml blood was draw into PAXgene tubes and total RNA extracted using Qiagen Rneasy mini kit following Qiagen protocol
|
| Label |
biotin
|
| Label protocol |
250ng total RNA was labeled using Ambion TotalPrep Illumina RNA amplification and labeling kit following vendor's protocol
|
| |
|
| Hybridization protocol |
1.5ug labeled cRNA from each sample was hybed to Illumina HumanWG-6 chip following Illumina protocol
|
| Scan protocol |
Illumina iScan protocol
|
| Description |
Data for Dr. Quan Li and Nancy Olsen's paper :Risk Factors for ANA Positivity in Healthy Persons
|
| Data processing |
data was processed using Illumina Genostudio software v2.0
version number of BeadStudio software and normalization algorithm: Module Gene Expression 3.3.8 and Quantile normalization.
|
| |
|
| Submission date |
Oct 14, 2010 |
| Last update date |
Oct 14, 2010 |
| Contact name |
yun lian |
| E-mail(s) |
yun.lian@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE24706 |
Gene Expression profiling of human PBMCs with different ANA titer |
|
