|
| Status |
Public on May 01, 2024 |
| Title |
Wildtype, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
Lung
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Epithelial cells Epithelial cells Epithelial cells
tissue: Lung
strain: Hopx-creERT2; R26R_EYFP
age: 8-10 weeks old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Target cells were FACS sorted and were loaded onto the 10x Chromium (10X Genomics) aiming for 10,000 cells per sample.
Libraries were prepared according to the manufacturer’s protocol using Chromium Single Cell 3’ v2 chemistry.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting were made using the STARSolo 2.7.9a and downstrema analysis perfimed using Seurat v4
Assembly: mm39
|
| |
|
| Submission date |
May 05, 2022 |
| Last update date |
May 01, 2024 |
| Contact name |
Michael Patrick Morley |
| E-mail(s) |
mmorley@pennmedicine.upenn.edu
|
| Phone |
215-898-2026
|
| Organization name |
Perelman School of Medicine at the University of Pennsylvania
|
| Department |
Penn Cardiovascular Institute
|
| Street address |
3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE202317 |
Single Cell sequencing analysis of AT1/AT2 cells from wildtype mouse, AT1-Cdc42 knockout mouse, and left bronchial ligation model |
| GSE202319 |
Mechanotransduction mediated by cyclical respiratory motion maintains lung alveolar epithelial cell fate |
|
| Relations |
| BioSample |
SAMN28102474 |
| SRA |
SRX15168205 |
