|
| Status |
Public on Jul 05, 2012 |
| Title |
Myotubes after 5 days in differentiating medium - Replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Myotubes 5 days in differentiating medium
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
genotype: wild type
tissue of origin: skeletal muscle
cell type: myotubes
time: 5 days
replicate: 3
|
| Growth protocol |
HAMS's F10 + 20% FBS + bFGF(4ng/ml) for the specified time (0h, 2d, 5d).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated in triplicates from mouse primary myoblasts and myotubes in growth and differentiation, respectively, using the RNeasy mini kit (Qiagen). The purity of RNA was analyzed by Bioanalyzer (Agilent Technologies, Santa Clara CA). All samples showed RNI > 9.0 and were used for subsequent labeling and hybridization.
|
| Label |
biotin
|
| Label protocol |
According to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
For microarray analysis, 100ng of total RNA was amplified and hybridized to the Mouse Gene 1.0 ST array.
|
| Scan protocol |
According to the standard Affymetrix protocol using an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
5D-3
|
| Data processing |
The data was RMA normalized from CEL file data per replicate set using GenePattern software from the Broad Institute.
|
| |
|
| Submission date |
Oct 19, 2010 |
| Last update date |
Jul 09, 2014 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE24811 |
Time Series of gene expression during the course of myogenic differentiation in mouse skeletal muscle cells |
| GSE24904 |
Snail regulates MyoD binding-site occupancy to direct enhancer switching and differentiation-specific transcription in myogenesis |
|
| Relations |
| Reanalyzed by |
GSE59272 |
