|
| Status |
Public on Sep 12, 2022 |
| Title |
JP8 |
| Sample type |
genomic |
| |
|
| Source name |
Mesenchymal stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease: HGPS-l (huntington gilford progeria syndrome like)
sentrix id: 203081450184
sentrix position: R07C01
|
| Growth protocol |
The hIPSc were differentiated into MSC, cultivated in KO DMEM supplemented 15% FBS, 10ng Beta FGF, 1mM ascobic acid 2-P, 1X glutamax, 1X NEAA, 50 µM Beta mercaptoethanol
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
macherey nagel nucleospin tissue kit
|
| Label |
biotin
|
| Label protocol |
According to Diagenode protocol
|
| |
|
| Hybridization protocol |
According to Diagenode protocol
|
| Scan protocol |
According to Diagenode protocol
|
| Data processing |
Raw IDAT files were processed using R package ChAMP. Probes with missing values are removed, samples with more than 10% of probes with a detection p-value of greater than 0.01, probes with a detection p-value of greater than 0.01 in at least one sample and probes for which 5% of samples have a bead count less than 3 are filtered out. Probes targeting CpG sites that are near SNPs, belong to X/Y chromosomes, or align with multiple locations are filtered out
|
| |
|
| Submission date |
May 06, 2022 |
| Last update date |
Sep 12, 2022 |
| Contact name |
Frederique Magdinier |
| Organization name |
Aix-Marseille Université - Marseille Medical Genetics
|
| Department |
MMG- U1251
|
| Street address |
27 Bvd Jean Moulin, Aix-Marseille Université. Faculté de Médecine. Campus La Timone
|
| City |
Marseille |
| ZIP/Postal code |
13005 |
| Country |
France |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE202368 |
Mesenchymal stem cells derived from patients with premature ageing syndromes display hallmarks of physiological ageing [array] |
| GSE202369 |
Mesenchymal stem cells derived from patients with premature ageing syndromes display hallmarks of physiological ageing |
|
