NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM612753 Query DataSets for GSM612753
Status Public on Sep 01, 2011
Title pMSG_72h_rep1 (microRNA)
Sample type RNA
 
Source name keratinocytes
Organism Homo sapiens
Characteristics cell type: skin keratinocyte
cell line: HaCaT
transfection: vector control
time: 72h
Treatment protocol E5 expression was induced by 1 mM dexamethasone treatment for different times.
Growth protocol HaCaT-E5 and control cells were grown in D-MEM growth medium with 10% FBS and glutamine and antibiotics, and serum-starved 24h before treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using mirVana miRNA Isolation kit (Ambion, Austin, TX) according to the manufacturer's instructions.
Label Cy3
Label protocol Total RNA was (100ng) was labeled with Cy3 -pCp following the instructions from the Agilent miRNA microarray system protocol (Agilent Technologies, Rockville, MD).
 
Hybridization protocol Hybridization was perfored following the instrcution in the Agilent miRNA microarray system protocol (Agilent Technologies, Rockville, MD). Hybridization was performed in +55 ºC for 20 hours. Washing was performed by the Agilent protocol 5 minutes in GE wash buffer 1 and 5 minutes in GE wash buffer 2 (+37ºC). Arrays were dried with a 1 minute spin in a Spectrafuge mini (Labnet, Woodbridge, NJ).
Scan protocol Default scanning performed with Axon 4200AL for Cy3 and Cy5 using resolution of five micrometer.
Description miRNA expression after 72h induction
Data processing log2 transformation followed by quantile normalization
 
Submission date Oct 25, 2010
Last update date Sep 01, 2011
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL6955
Series (1)
GSE24908 Human Papillomavirus 16 E5 modulates the expression of host microRNAs

Data table header descriptions
ID_REF
VALUE quantile normalized log2 median foreground intensities

Data table
ID_REF VALUE
1399 9.538178595
2921 9.586244395
3382 9.585288255
5933 9.49056828
7480 9.581189814
10453 9.558221412
10472 9.532756248
11306 9.445712528
11857 9.559358372
15386 9.405872136
1672 9.492176717
2668 9.479116526
3035 9.463323871
3192 9.483236306
10400 9.49326534
14445 9.590748961
14576 9.433493312
14693 9.508164769
14755 9.48425108
15284 9.569456235

Total number of rows: 11080

Table truncated, full table size 185 Kbytes.




Supplementary file Size Download File type/resource
GSM612753_pMSG_72h_rep1.gpr.gz 1.0 Mb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.