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Sample GSM613377 Query DataSets for GSM613377
Status Public on Dec 28, 2012
Title Patient1_PBMC_rep1
Sample type RNA
 
Source name Patient1, PBMC, replicate 1
Organism Homo sapiens
Characteristics cell type: PBMC
gender: Male
age (y): 39
disease state: Influenza A (H1N1)
Extracted molecule total RNA
Extraction protocol Blood samples were collected in EDTA-treated tubes and PBMCs were isolated by standard Ficoll density-gradient centrifugation. PBMCs were washed once in sterile phosphate-buffered saline (PBS) before RNA isolation. Total RNA was isolated from freshly obtained PBMCs using the mirVana miRNA PARIS kit (Ambion), in accordance with the manufacture’s protocol.
Label Cy3
Label protocol Samples of total RNA were labeled and hybridized with the miRNA Labeling Reagent and Hybridization Kit (Agilent, Santa Clara, CA USA) according to the manufacturer’s protocol.
 
Hybridization protocol Samples of total RNA were labeled and hybridized with the miRNA Labeling Reagent and Hybridization Kit (Agilent, Santa Clara, CA USA) according to the manufacturer’s protocol. After hybridization, microarrays were washed 5 minute at room temperature with GE Wash Buffer 1 (Agilent) and 5 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent Microarray Scanner using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 5um, Dye channel is set to Green(PMT100, PMT5).
Description critically ill Patient with Influenza A (H1N1)
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
 
Submission date Oct 27, 2010
Last update date Dec 28, 2012
Contact name Hao Song
E-mail(s) songhao@nwsuaf.edu.cn
Organization name Northwest A & F University
Department College of Veterinary Medicine
Lab Research Laboratory of Virology, Immunology & Bioinformatics
Street address NO.3 Xinong Road,Yangling
City Xi’an
State/province Shaanxi Province
ZIP/Postal code 712100
Country China
 
Platform ID GPL10850
Series (1)
GSE24956 Microarray Analysis of MicroRNA Expression in Peripheral Blood Mononuclear Cells of critically ill Patients with Influenza A (H1N1)

Data table header descriptions
ID_REF
VALUE background subtracted and spatially detrended Processed Signal intensities

Data table
ID_REF VALUE
miRNABrightCorner30 7.678144
DarkCorner 2.7789462
hsa-miR-720 13.910403
hsa-miR-587 0.007525928
hsa-miR-1236 1.7044951
hsa-miR-607 0.007525928
hsa-miR-1253 0.007525928
hsa-miR-369-3p 0.464735
hsa-miR-657 0.007525928
hsa-miR-526b 0.007525928
hsa-miR-515-3p 0.007525928
hsa-miR-586 0.007525928
hsa-miR-485-5p 0.007525928
hsa-miR-507 0.007525928
hsa-miR-551b* 0.007525928
hsa-miR-326 4.999502
hsa-miR-146b-3p 0.007525928
hsa-miR-1283 0.007525928
hsa-miR-622 0.007525928
hsa-miR-556-5p 0.007525928

Total number of rows: 961

Table truncated, full table size 22 Kbytes.




Supplementary file Size Download File type/resource
GSM613377_HE-1US80803205_252182712365.txt.gz 2.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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