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Status |
Public on Dec 28, 2012 |
Title |
Patient1_PBMC_rep1 |
Sample type |
RNA |
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|
Source name |
Patient1, PBMC, replicate 1
|
Organism |
Homo sapiens |
Characteristics |
cell type: PBMC gender: Male age (y): 39 disease state: Influenza A (H1N1)
|
Extracted molecule |
total RNA |
Extraction protocol |
Blood samples were collected in EDTA-treated tubes and PBMCs were isolated by standard Ficoll density-gradient centrifugation. PBMCs were washed once in sterile phosphate-buffered saline (PBS) before RNA isolation. Total RNA was isolated from freshly obtained PBMCs using the mirVana miRNA PARIS kit (Ambion), in accordance with the manufacture’s protocol.
|
Label |
Cy3
|
Label protocol |
Samples of total RNA were labeled and hybridized with the miRNA Labeling Reagent and Hybridization Kit (Agilent, Santa Clara, CA USA) according to the manufacturer’s protocol.
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|
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Hybridization protocol |
Samples of total RNA were labeled and hybridized with the miRNA Labeling Reagent and Hybridization Kit (Agilent, Santa Clara, CA USA) according to the manufacturer’s protocol. After hybridization, microarrays were washed 5 minute at room temperature with GE Wash Buffer 1 (Agilent) and 5 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
Scan protocol |
Slides were scanned immediately after washing on the Agilent Microarray Scanner using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 5um, Dye channel is set to Green(PMT100, PMT5).
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Description |
critically ill Patient with Influenza A (H1N1)
|
Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
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|
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Submission date |
Oct 27, 2010 |
Last update date |
Dec 28, 2012 |
Contact name |
Hao Song |
E-mail(s) |
songhao@nwsuaf.edu.cn
|
Organization name |
Northwest A & F University
|
Department |
College of Veterinary Medicine
|
Lab |
Research Laboratory of Virology, Immunology & Bioinformatics
|
Street address |
NO.3 Xinong Road,Yangling
|
City |
Xi’an |
State/province |
Shaanxi Province |
ZIP/Postal code |
712100 |
Country |
China |
|
|
Platform ID |
GPL10850 |
Series (1) |
GSE24956 |
Microarray Analysis of MicroRNA Expression in Peripheral Blood Mononuclear Cells of critically ill Patients with Influenza A (H1N1) |
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