|
| Status |
Public on Dec 23, 2011 |
| Title |
3d replicate 2 (miRNA) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
dissected brain tissue
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dissected brain
time: 3d
|
| Treatment protocol |
natural aging
|
| Growth protocol |
Fly were grown in standard cornmeal molasses agar medium with dry yeast, at 25°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion miRvana miRNA isolation kit
|
| Label |
Hy3
|
| Label protocol |
miRCURY Labeling Kit (Exiqon) for use with Exiqon miRNA arrays
|
| |
|
| Channel 2 |
| Source name |
common reference pool
|
| Organism |
Drosophila melanogaster |
| Characteristics |
common reference: pooled RNA from all samples
|
| Treatment protocol |
natural aging
|
| Growth protocol |
Fly were grown in standard cornmeal molasses agar medium with dry yeast, at 25°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion miRvana miRNA isolation kit
|
| Label |
Hy5
|
| Label protocol |
miRCURY Labeling Kit (Exiqon) for use with Exiqon miRNA arrays
|
| |
|
| |
| Hybridization protocol |
Tecan Hybridization with Exiqon Hybridization Program
|
| Scan protocol |
Axon Instruments 4000B Scanner using GenePix Pro 6.0 image acquisition software
|
| Description |
small RNA profiling
miRNA0526H 3 Days #2 (Hy3) vs Pool (Hy5) 050407.gpr
|
| Data processing |
Global LOESS regression algorithm (locally weighted scatterplot smoothing).
~80 array features listed below are meaningful data for Drosophila miRNAs, all others are either unrelated sequences or human miRNA sequences that Exiqon used for test/development of new products. So we consider only those 80 or so probesets are useful.
|
| |
|
| Submission date |
Oct 28, 2010 |
| Last update date |
Jan 23, 2015 |
| Contact name |
Bonini Nancy |
| Organization name |
University of Pennsylvania
|
| Street address |
415 S University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL11139 |
| Series (2) |
| GSE24992 |
Drosophila brain microRNA expression with age: miRNA profiling |
| GSE25009 |
Drosophila miR-34 mechanistically links aging and neurodegeneration |
|
