|
| Status |
Public on Dec 23, 2011 |
| Title |
60d replicate 2 (mRNA) |
| Sample type |
RNA |
| |
|
| Source name |
dissected brain tissue
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dissected brain
time: 60d
|
| Treatment protocol |
natural aging
|
| Growth protocol |
Fly were grown in standard cornmeal molasses agar medium with dry yeast, at 25°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent
|
| Label |
biotin
|
| Label protocol |
FL-Ovation™ cDNA Biotin Module V2 (Ovation)
|
| |
|
| Hybridization protocol |
Affymetrix Drosophila 2.0 chips. Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
HHMI (640) 60 days #2 Drosophila_2.CEL
HHMI (640) 60 days #2 Drosophila_2.CHP
|
| Data processing |
Affymetrix .cel (probe intensity) files were exported from GeneChip Operating Software (Affymetrix). The .cel files were imported to ArrayAssist Lite (Agilent) in which GCRMA probeset expression levels and Affymetrix absent/present/marginal flags were calculated.
MAS5.0-generated data are presented in the tables below.
|
| |
|
| Submission date |
Oct 28, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Bonini Nancy |
| Organization name |
University of Pennsylvania
|
| Street address |
415 S University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE25007 |
Drosophila brain gene expression with age: mRNA profiling |
| GSE25009 |
Drosophila miR-34 mechanistically links aging and neurodegeneration |
|
| Relations |
| Reanalyzed by |
GSE119084 |
