|
| Status |
Public on Dec 20, 2010 |
| Title |
K41 NoDox-3 |
| Sample type |
RNA |
| |
|
| Source name |
differentiated mouse ES cells 4155448007 E
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129P2 (also referred to as 129/Ola)
cell type: K41
doxycycline: no
|
| Treatment protocol |
Doxycycline treatment was performed for 6 hours on day 6
|
| Growth protocol |
EBs were differentiated until day 6. Endothelial (FV) or Hematopoietic (K41) progenitors cells were sorted by facs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Trizol protocol
|
| Label |
biotin
|
| Label protocol |
Standard Illumina label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
K41 NoDox-3
|
| Data processing |
The data were normalised using cubic spline normalisation with BeadStudio then median normalisation with GeneSpring
|
| |
|
| Submission date |
Nov 02, 2010 |
| Last update date |
Dec 20, 2010 |
| Contact name |
Michael Kyba |
| Organization name |
University of Minnesota
|
| Lab |
Kyba Lab
|
| Street address |
312 Church St. SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL6238 |
| Series (2) |
| GSE25080 |
Genes regulated by HoxA3 in endothelial and hematopoietic progenitors |
| GSE25096 |
HoxA3 is an apical regulator of hemogenic endothelium |
|
