|
Status |
Public on Jun 15, 2023 |
Title |
Cpa3Cre/+, immunized, challenge day 1, rep2 |
Sample type |
SRA |
|
|
Source name |
intestine
|
Organism |
Mus musculus |
Characteristics |
genotype: Cpa3Cre/+ tissue: intestine Sex: m treatment: 2mg alum + 25 µg ovalbumin i.p. on day 0 and 14 + 2 bottle test (water vs. Water + 20% egg white + 8% sugar) on day 20 time: day 1
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from whole Jejunum (fat and Peyer's plaques removed) according to the instructions of the PureLink RNA Mini kit (Invitrogen) RNA libraries for RNA-seq were prepared using TruSew Stranded RNA Kit (Illumina) following manufacturer's protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 1000 |
|
|
Description |
AS-631210-LR-56766
|
Data processing |
reads were aligned via STAR aligner (default settings, v.2.5.2b) and counted via FeatureCount (default settings, stranded library, subread/1.5.1) Assembly: mm10 (Mus_musculus_GRCm38_102) Supplementary files format and content: text delimeted file includes raw counts for each sample
|
|
|
Submission date |
May 17, 2022 |
Last update date |
Jun 15, 2023 |
Contact name |
Robin Thiele |
E-mail(s) |
robin.thiele@dkfz-heidelberg.de
|
Organization name |
German Cancer Research Center
|
Department |
Immunology
|
Lab |
Cellular Immunology
|
Street address |
Im Neuenheimer Feld 280
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL32159 |
Series (1) |
GSE203209 |
Intellicage analysis of Cpa3+/+ and Cpa3Cre mice |
|
Relations |
BioSample |
SAMN28495416 |
SRA |
SRX15312175 |