|
| Status |
Public on Nov 16, 2010 |
| Title |
Sarda, 2nd animal |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Reference Sheep (Sarda)
|
| Organism |
Ovis aries |
| Characteristics |
breed: Sarda
gender: female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI) extraction protocol
|
| Label |
Cy5
|
| Label protocol |
NimbleGen Dual-Color DNA Labeling Kit
|
| |
|
| Channel 2 |
| Source name |
Test Sheep (Sarda, animal 2)
|
| Organism |
Ovis aries |
| Characteristics |
breed: Sarda
gender: female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI) extraction protocol
|
| Label |
Cy3
|
| Label protocol |
NimbleGen Dual-Color DNA Labeling Kit
|
| |
|
| |
| Hybridization protocol |
NimbleGen Hybridization Kit
|
| Scan protocol |
NimbleGen MS 200 Microarray Scanner with default parameters
|
| Data processing |
Data normalization was conducted using the normalize.qsline method from the Bioconductor package in R. Then data were analysed for each hybridization using normalized log2 ratios using the CGHweb server.
|
| |
|
| Submission date |
Nov 03, 2010 |
| Last update date |
Nov 15, 2010 |
| Contact name |
Pier Luigi Martelli |
| E-mail(s) |
gigi@biocomp.unibo.it
|
| URL |
http://www.biocomp.unibo.it
|
| Organization name |
University of Bologna
|
| Street address |
via Irnerio 42
|
| City |
Bologna |
| ZIP/Postal code |
40126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10990 |
| Series (1) |
| GSE25122 |
A first comparative map of copy number variations in the sheep genome |
|
