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Sample GSM618421 Query DataSets for GSM618421
Status Public on Feb 07, 2011
Title cycle 14B female
Sample type SRA
 
Source name embryo
Organism Drosophila melanogaster
Characteristics paternal strain: Canton-S
maternal strain: His2Av-mRFP III.1 (Bloomington 23650)
mitotic cycle: 14 (25-50% membrane envagination)
Growth protocol embryos were collected from crosses of CantonS females and w1 males, imaged live under a fluorescent scope and harvested into Trizol at desired stage
Extracted molecule total RNA
Extraction protocol libraries were prepared according to a version of Illumina's mRNA-Seq protocol adapted for small sample inputs; sex was determined by genotyping genomic DNA from analyzed embryos
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer II
 
Description mRNA isolated from single embryo
instrument model: Illumina Genome Analyzer IIx
Data processing alignment: TopHat v1.0.13 using default settings
expression levels: Cufflinks v0.8.2 and Cuffcompare using default settings
 
Submission date Nov 07, 2010
Last update date May 15, 2019
Contact name Michael Eisen
E-mail(s) mbeisen@berkeley.edu
Phone 510-666-3639
Organization name University of California, Berkeley
Department Molecular and Cell Biology
Lab Eisen Lab
Street address Stanley Hall 304B
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platform ID GPL9061
Series (1)
GSE25180 Non-canonical compensation of zygotic X transcription in Drosophila melanogaster development revealed through single embryo RNA-Seq
Relations
SRA SRX031149
BioSample SAMN00120310

Supplementary file Size Download File type/resource
GSM618421_F14B.bedgraph.gz 29.1 Mb (ftp)(http) BEDGRAPH
GSM618421_F14B.cuff.gz 219.3 Kb (ftp)(http) CUFF
GSM618421_F14B.sam.gz 326.5 Mb (ftp)(http) SAM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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