|
Status |
Public on Feb 07, 2011 |
Title |
cycle 14B female |
Sample type |
SRA |
|
|
Source name |
embryo
|
Organism |
Drosophila melanogaster |
Characteristics |
paternal strain: Canton-S maternal strain: His2Av-mRFP III.1 (Bloomington 23650) mitotic cycle: 14 (25-50% membrane envagination)
|
Growth protocol |
embryos were collected from crosses of CantonS females and w1 males, imaged live under a fluorescent scope and harvested into Trizol at desired stage
|
Extracted molecule |
total RNA |
Extraction protocol |
libraries were prepared according to a version of Illumina's mRNA-Seq protocol adapted for small sample inputs; sex was determined by genotyping genomic DNA from analyzed embryos
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer II |
|
|
Description |
mRNA isolated from single embryo instrument model: Illumina Genome Analyzer IIx
|
Data processing |
alignment: TopHat v1.0.13 using default settings expression levels: Cufflinks v0.8.2 and Cuffcompare using default settings
|
|
|
Submission date |
Nov 07, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Michael Eisen |
E-mail(s) |
mbeisen@berkeley.edu
|
Phone |
510-666-3639
|
Organization name |
University of California, Berkeley
|
Department |
Molecular and Cell Biology
|
Lab |
Eisen Lab
|
Street address |
Stanley Hall 304B
|
City |
Berkeley |
State/province |
CA |
ZIP/Postal code |
94720 |
Country |
USA |
|
|
Platform ID |
GPL9061 |
Series (1) |
GSE25180 |
Non-canonical compensation of zygotic X transcription in Drosophila melanogaster development revealed through single embryo RNA-Seq |
|
Relations |
SRA |
SRX031149 |
BioSample |
SAMN00120310 |