|
| Status |
Public on Mar 09, 2012 |
| Title |
TM35 |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer
|
| Organism |
Homo sapiens |
| Characteristics |
age: 52
histotype: serous
Stage: III
grading: 3
debulking: mRD
therapy code: P
end point: early
ttr code: 1
ttr (months): 1
os code: 1
overall survival (months): 12
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For total RNA extraction, approximately 100 mg of frozen tissue was homogenized using a bench-top homogenizer (MM200, Retsch, Germany) in 1 mL of TRIzol Reagent (Invitrogen). Subsequent miRNA extraction was performed using the miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 750 ng total RNA using the high-throughput Gene expression profiling, the DASL Assay (cDNA-mediated annealing, selection, extension and ligation)
|
| |
|
| Hybridization protocol |
Fluorescently labelled cDNA PCR products were hybridized to the Illumina Sentrix BeadChip according to the protocols provided by the manufacturer.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Array data export processing and analysis was performed using Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). The data were normalised using quantile normalisation.
|
| |
|
| Submission date |
Nov 08, 2010 |
| Last update date |
Apr 17, 2015 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
|
| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8179 |
| Series (2) |
| GSE25202 |
microRNA alterations associated to clinical response in advanced stage ovarian cancer patients (training set) |
| GSE25204 |
microRNA alterations associated to clinical response in advanced stage ovarian cancer patients |
|
