|
| Status |
Public on Jul 06, 2011 |
| Title |
beta Galactosidase rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Xenopus juvenile frog whole brain
|
| Organism |
Xenopus laevis |
| Characteristics |
strain: periodic albino
developmental stage: juvenile
tissue: brain
chip antibody: control antibody
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The complete mRNP IP protocol is provided as a supplementary file on the Series record (GSE25207_IP_of_mRNP_complexes_protocol.txt).
RNA from Immunoprecipitated samples was isolated by phenol chloroform extraction and further purified on Qiagen Rneasy columns before performing quality control analysis on Agilent Bioanalyzer chips.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol for Exon arrays.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol for Exon arrays.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner.
|
| Description |
anti-beta galactosidase co-immunoprecipitated RNAs
Gene expression data of control antibody, co-immunoprecipitated RNAs from juvenile frog brain
|
| Data processing |
Raw data were corrected for background and transcripts scored as present or absent, using MAS 5.0 and further filtered to remove the bottom 30th percentile (GeneSpring GX 10).
|
| |
|
| Submission date |
Nov 08, 2010 |
| Last update date |
Jul 06, 2011 |
| Contact name |
Ben Gregory Szaro |
| E-mail(s) |
bszaro@albany.edu
|
| Phone |
518-591-8852
|
| Organization name |
University at Albany, State University of New York
|
| Department |
Biological Sciences
|
| Lab |
Life Sciences Research 1059
|
| Street address |
1400 Washington Avenue
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE25207 |
RIP-Chip Analysis of hnRNP K in Xenopus juvenile brain |
|
