|
| Status |
Public on Nov 10, 2010 |
| Title |
Cryptorchid cremasteric sac, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse cremasteric sac, cryptorchid
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed
genotype: Tg(shRxfp2)
phenotype: cryptorchidism
gender: male
age: 1 month
tissue: cremasteric sac
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Replicate 2.
|
| Data processing |
The data were normalized using quantile normalization with GenomeStudioV2010.2 Gene expression module V1.7.0.
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Nov 10, 2010 |
| Contact name |
Alexander I Agoulnik |
| E-mail(s) |
aagoulni@fiu.edu
|
| Organization name |
Florida International University
|
| Department |
Herbert Wertheim College of Medicine
|
| Street address |
11200 SW 8th Street, HLSI 419B
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33199 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE25222 |
Suppression of insulin like3 receptor reveals the role of β-catenin and Notch signaling in gubernaculum development |
|
