Following animal euthanasia, samples of all wounds were frozen in RNAlater (Ambion, Austin, TX) at -80oC for molecular analysis. The epithelium of all incisional grid samples was mechanically separated from the underlying mesenchymal tissue using a single-edged razor blade (Schierle et al. 2007), and was homogenized in RLT buffer (Qiagen, Valencia, CA) with 1% beta- mercaptoethanol using a PowerGen Model 500 Homogenizer (Fisher Scientific, Houston, TX). Total RNA was extracted and purified using the RNeasy kit (Qiagen, Valencia, CA) as previously described (Reno et al. 1997). RNA quality was determined using the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label
Cy3
Label protocol
standard as recommended by manufacturer
Hybridization protocol
standard as recommended by manufacturer
Scan protocol
standard as recommended by manufacturer
Description
Tissue is cutaneous, measured time is at 5d
Data processing
We used quantile normalization and Bioconductor package Agi4x44Preprocess version 1.6.0 to process the data.
