|
| Status |
Public on Nov 17, 2010 |
| Title |
Hec50_neg_rep_3 |
| Sample type |
RNA |
| |
|
| Source name |
scrambled negative control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hec50
cell type: endometrial cancer
|
| Treatment protocol |
Cells were treated with 60 nM miR-200c mimic or scrambled negative control (Applied Biosystems) for 48 hrs prior to extraction
|
| Growth protocol |
Cells were grown in DMEM supplemented with L-glutamine and 10% FBS in an incubator at 37 degrees and 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's directions. RNA integrity was confirmed using an Agilent Bioanalyzer (Agilent, Palo Alto, CA)
|
| Label |
biotin
|
| Label protocol |
Labeled complementary RNA was made using the GeneChip_IVT labeling kit (Affymetrix, Inc., Santa Clara, CA)
|
| |
|
| Hybridization protocol |
20 ug biotin-labeled cDNA was fragmented and hybridized.
|
| Scan protocol |
Microarray analysis was performed using Affymetrix gene chips (HuFL-U133 plus2) and scanned using Affymetrix Genechip Scanner 3000
|
| Description |
Endometrial cancer cell line (Hec50) mock transfected, or transfected with scrambled negative control or miR-200c
|
| Data processing |
All entities: Data normalized to chip. 1_5 fold 200c vs mock and neg: Data was analyzed using Genespring GX 9.0 (Agilent). Data was filtered using a 1.5 fold change cutoff and a p value of 0.05 (ANOVA, with Benjamini Hochberg FDR multiple testing correction).
|
| |
|
| Submission date |
Nov 15, 2010 |
| Last update date |
Nov 17, 2010 |
| Contact name |
Erin N. Howe |
| E-mail(s) |
erin.howe@ucdenver.edu
|
| Organization name |
University of Colorado at Denver, Anschutz Medical Campus
|
| Department |
Pathology
|
| Lab |
Richer Lab
|
| Street address |
UCD AMC, Mail Stop 8104 PO Box 6511
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE25332 |
Restoring miR-200c to aggressive endometrial cancer cell line |
|
