|
| Status |
Public on Jun 13, 2022 |
| Title |
LMZ RA-depleted mid-gastrula, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
GFP-Cyp26-injected Xenopus embryo
|
| Organism |
Xenopus laevis |
| Characteristics |
developmental stage: Mid-gastrula stage
treatment: GFP-Cyp26-injected
|
| Treatment protocol |
Control RNA mix (GFP) or RA-depletion mix (GFP and cyp26) are injected equatorially into the 4 blastomeres at the 4-cell stage
|
| Growth protocol |
LMZ explants are dissected at early gastrula stage from embryos previously injected at the 4-cell stage. Explants are collected 1h, 3h after dissection (culture 20°C) and overnight (culture 14)C)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quiagen RNeasy kit according to manufacturer instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2005-2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Xenopus laevis Genome 2.0 Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the ThermofisherGeneChip Scanner 3000 7G.
|
| Description |
Gene expression from lateral marginal zone explants at the origin of lateral mesoderm and pronephric kidney field
|
| Data processing |
CEL files were read and converted to an expression measure using RMA in R (justRMA function of Affy R package)
|
| |
|
| Submission date |
Jun 10, 2022 |
| Last update date |
Jun 13, 2022 |
| Contact name |
Jean-Francois RIOU |
| E-mail(s) |
jean-francois.riou@sorbonne-universite.fr
|
| Organization name |
CNRS/Sorbonne Universite
|
| Department |
Institut de Biologie Paris-Seine
|
| Lab |
Biologie du Developpement UMR7622
|
| Street address |
9, quai Saint-Bernard, case 24
|
| City |
PARIS |
| ZIP/Postal code |
75252 cedex 05 |
| Country |
France |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE205827 |
Expression profiling of retinoic acid targets in lateral mesoderm from Xenopus laevis embryos |
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