|
| Status |
Public on Mar 06, 2023 |
| Title |
Prepubertal_SUS_testis1_B5 |
| Sample type |
SRA |
| |
|
| Source name |
Testis
|
| Organism |
Sus scrofa |
| Characteristics |
cell type: Total testicular cells
tissue: Testis
strain: mixed breed
age: 3 months old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Testes from pre-pubertal Holstein breed cattle and mixed breed pigs were collected by castration. For all samples, single-cell suspensions of testicular parenchyma were generated as described previously (Oatley and Brinster, 2006; Oatley et al., 2016).
Live cells (~8000 per sample) were then loaded into a Chromium Controller (10X Genomics, Inc.), and cDNA libraries were generated using v2 chemistry kits (10X Genomics, Inc.). Libraries were pooled at proportions netting equal read depth and sequenced in a single lane on an Illumina HiSeq 4000 (Genomics and Cell Characterization Core Facility, University of Oregon).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
Raw base call files were demultiplexed using the 10X Genomics Cell Ranger pipeline (v2.1.0)
Assembly: Bovine genome (ARS-UCD1.2) or porcine genome (Sscrofa11.1).
Supplementary files format and content: Barcodes (.tsv and .gz), Features(.tsv and .gz) and matrix (.mtx)
|
| |
|
| Submission date |
Jun 15, 2022 |
| Last update date |
Mar 07, 2023 |
| Contact name |
Jon Oatley |
| E-mail(s) |
joatley@wsu.edu
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Lab |
Oatley Lab
|
| Street address |
202 Biotechnology Life Science Building PO Box 647520,
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164-7520 |
| Country |
USA |
| |
|
| Platform ID |
GPL22475 |
| Series (1) |
| GSE206156 |
The evolutionarily conserved gene Arrdc5 is required for male fertility in mice |
|
| Relations |
| BioSample |
SAMN29068899 |
| SRA |
SRX15711664 |
