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Sample GSM6249328 Query DataSets for GSM6249328
Status Public on Aug 08, 2022
Title CNTO1275UCO3001-100081 colon biopsy week 0
Sample type RNA
 
Source name colon biopsy
Organism Homo sapiens
Characteristics tissue: colon
donor id: CNTO1275UCO3001-100081
visit: WEEK I-0
diagnosis: ulcerative colitis
treatment: Placebo IV
mucosal healing at week 8: Y
clinical remission at week 8: Y
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
Label biotin
Label protocol Target was prepared from 100ng total RNA using the NuGEN Ovation V2 kit, followed by NuGEN Encore biotin labeling module, using the manufacturer’s protocol.
 
Hybridization protocol Following fragmentation, 1.875ug of cDNA was hybridized for 16 hr at 45oC on GeneChip HT HG-U133+ PM 96 array plates. GeneChips were washed and stained in the Affymetrix GeneChip Array Station.
Scan protocol GeneChips were scanned using the GeneChipHT Scanner.
Data processing The data was normalized by RMA using ArrayStudio version 10.
 
Submission date Jun 16, 2022
Last update date Aug 08, 2022
Contact name Nick Powell
E-mail(s) npowell@ic.ac.uk
Organization name Imperial College London
Department Department of Metabolism, Digestion and Reproduction
Street address Du Cane Road
City London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platform ID GPL13158
Series (1)
GSE206285 Efficacy and safety of ustekinumab treatment in patients with ulcerative colitis

Data table header descriptions
ID_REF
VALUE log2 RMA signal.

Data table
ID_REF VALUE
AFFX-BioB-5_at 9.0033
AFFX-BioB-M_at 9.2018
AFFX-BioB-3_at 9.1035
AFFX-BioC-5_at 10.2106
AFFX-BioC-3_at 9.3872
AFFX-BioDn-5_at 11.1793
AFFX-BioDn-3_at 11.9957
AFFX-CreX-5_at 12.8277
AFFX-CreX-3_at 13.0098
AFFX-DapX-5_at 3.1834
AFFX-DapX-M_at 2.9861
AFFX-DapX-3_at 2.8889
AFFX-LysX-5_at 2.9419
AFFX-LysX-M_at 2.8702
AFFX-LysX-3_at 2.9647
AFFX-PheX-5_at 3.3897
AFFX-PheX-M_at 3.0656
AFFX-PheX-3_at 3.9804
AFFX-ThrX-5_at 3.394
AFFX-ThrX-M_at 3.1345

Total number of rows: 54715

Table truncated, full table size 1107 Kbytes.




Supplementary file Size Download File type/resource
GSM6249328_JNJ_WO136_6201675643-06_G1_P16_G01_HT_HG-U133_Plus_PM_G01.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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