|
| Status |
Public on Aug 08, 2022 |
| Title |
CNTO1275UCO3001-100393 colon biopsy week 0 |
| Sample type |
RNA |
| |
|
| Source name |
colon biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colon
donor id: CNTO1275UCO3001-100393
visit: WEEK I-0
diagnosis: ulcerative colitis
treatment: Ustekinumab 130 mg IV
mucosal healing at week 8: Y
clinical remission at week 8: Y
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Target was prepared from 100ng total RNA using the NuGEN Ovation V2 kit, followed by NuGEN Encore biotin labeling module, using the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.875ug of cDNA was hybridized for 16 hr at 45oC on GeneChip HT HG-U133+ PM 96 array plates. GeneChips were washed and stained in the Affymetrix GeneChip Array Station.
|
| Scan protocol |
GeneChips were scanned using the GeneChipHT Scanner.
|
| Data processing |
The data was normalized by RMA using ArrayStudio version 10.
|
| |
|
| Submission date |
Jun 16, 2022 |
| Last update date |
Aug 08, 2022 |
| Contact name |
Nick Powell |
| E-mail(s) |
npowell@ic.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Department of Metabolism, Digestion and Reproduction
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE206285 |
Efficacy and safety of ustekinumab treatment in patients with ulcerative colitis |
|
