|
| Status |
Public on Feb 15, 2023 |
| Title |
P65c2E_HIPCTX |
| Sample type |
SRA |
| |
|
| Source name |
Forebrain
|
| Organism |
Mus |
| Characteristics |
tissue: Forebrain
developmental stage: Postnatal day 6
Sex: Male
genotype: Kdm5c hemizygous deletion
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
total RNA was purified by Qiagen RNEasy Midi Kit (75144)
cDNA library was generated from purified total RNA and prepared by poly-A enrichment
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Mapped reads to mus musculus mm10 Gencode genome through STAR (v2.5.3a)
Counts file generated by featureCounts (Subread v1.5.0)
BAM files were convered to bigwigs using deeptools (v3.1.3) and visualized by the UCSC genome browser
Assembly: mm10
Supplementary files format and content: bigwig
Supplementary files format and content: counts table
|
| |
|
| Submission date |
Jun 17, 2022 |
| Last update date |
Feb 15, 2023 |
| Contact name |
Shigeki Iwase |
| E-mail(s) |
siwase@umich.edu, kbonefas@umich.edu
|
| Organization name |
University of Michigan
|
| Lab |
Iwase Lab
|
| Street address |
1241 E. Catherine St.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL31136 |
| Series (1) |
| GSE206346 |
Sexually Dimorphic Alterations in the Transcriptome and Behavior with Loss of Histone Demethylase KDM5C |
|
| Relations |
| BioSample |
SAMN29176691 |
| SRA |
SRX15780238 |
