|
| Status |
Public on Jun 26, 2022 |
| Title |
mCRC_036 |
| Sample type |
RNA |
| |
|
| Source name |
tumour tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Female
date of birth: 1940-08-15
date of diagnosis: 2017-04-10
age of diagnosis: 77
tumour localization: Sigma
localization (right or left): left
state at diagnosis: IV
primary surgery: No
date of surgery: NA
adjuvancy: No
tipe of adjuvancy: 0
metastasis: 2017-04-05
liver metastasis: Yes
number of liver metastasis: 3
extrahepatic metastasis: Yes
lung metastasis: Yes
peritoneal metastases: No
metastatic lymph nodes: No
metastasis in other: No
number of affected organs: 2
ps: 2
ldh: 1046
ldh>675: 1
ldh>450: 1
f.alc.: 640
f.alc>116: 1
leukocytes: 16180
pcr: 10.2
pcr>1: Yes
treatment: FOLFOX
response: Not evaluated
progression: 1
date of progression: 2017-05-16
treatment at progression: 0
status: Dead
date of last follow up: 2017-05-16
mutations: KRAS, TP53
genotype: RAS_mut
number of mutations: 2
mss: MSS
gemcad classification: high
|
| Extracted molecule |
total RNA |
| Extraction protocol |
High Pure FFPET RNA isolation kit, Roche
|
| Label |
n.a.
|
| Label protocol |
pathology of study (mCRC) and the order to RNA extraction
|
| |
|
| Hybridization protocol |
Gene Expression RNA CodeSet Hybridization Protocol MAN-10056: Prepare Master Mix adding 70uL of Hybridization Buffer to the tube containing the 42uL of Reporter CodeSet. Add 8 μL of Master Mix to each well of a strip tube, Add 5 µL of sample to each tube containing Master Mix. Mix the Capture ProbeSet tube by inverting or flicking, and briefly spin down the contents. Add 2 μL of Capture ProbeSet to each tube. Cap the strip tubes tightly and mix. Spin briefly and immediately place the tubes in a pre-heated 65°C thermal cycler. Incubate hybridization reactions for 16 hours. Incubate at 4°C and read.
|
| Scan protocol |
nCounter Prep Station using nCounter Analysis System User Manual (MAN-C0035), nSolver software
|
| Data processing |
The raw data were analyzed with nSolver software. Only the samples that passed the control quality were normalized by nSolver, using default settings and taking into account the positive and negative probes and the housekeeping genes in the panel
|
| |
|
| Submission date |
Jun 21, 2022 |
| Last update date |
Jun 26, 2022 |
| Contact name |
Leire Pedrosa |
| E-mail(s) |
LEPEDROSA@recerca.clinic.cat
|
| Organization name |
IDIBAPS
|
| Street address |
Carrer del Villarroel 170
|
| City |
BARCELONA |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL32069 |
| Series (1) |
| GSE206613 |
A novel gene signature unveils three distinct immune-metabolic rewiring patterns conserved across diverse tumor types and associated with outcome |
|
