|
| Status |
Public on Apr 30, 2011 |
| Title |
Exposed_AC 5 |
| Sample type |
RNA |
| |
|
| Source name |
Lung Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
agent: asbestos
disease state: AC
tissue: lung tumor
asbestos-fiber count: 10.8
|
| Growth protocol |
The tissues were maintained at -70°C prior to extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed by using the miRNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Agilent’s miRNA Complete Labeling and Hybridization Kit V2 (Protocol Version 2.1, Agilent Technologies, Palo Alto, CA, USA) was utilized for processing the samples.
|
| |
|
| Hybridization protocol |
Agilent’s miRNA Complete Labeling and Hybridization Kit V2 (Protocol Version 2.1, Agilent Technologies, Palo Alto, CA, USA) was utilized for processing the samples.
|
| Scan protocol |
High-resolution Microarray Scanner (G2565CA, Agilent Technologies, Palo Alto, CA, USA) was used to scan the microarrays and the images were processed with Agilent Feature Extraction Software v. 9.5.
|
| Description |
asbestos-fiber_count 10.8
|
| Data processing |
Images were processed with Agilent Feature Extraction Software v. 9.5. The data was analyzed using Chipster version 1.4.2 (http://chipster.csc.fi/).
|
| |
|
| Submission date |
Nov 19, 2010 |
| Last update date |
Apr 30, 2011 |
| Contact name |
Mohamed Guled |
| E-mail(s) |
mohamed.guled@helsinki.fi
|
| Organization name |
Haartman Institute, University of Helsinki
|
| Department |
Department of Pathology
|
| Lab |
CMG-Lab
|
| Street address |
Haarmaninkatu 3
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL7731 |
| Series (1) |
| GSE25508 |
Integrative Analysis of microRNA, mRNA and DNA Copy Number Data Reveals Asbestos-Related Changes in Non-Small Cell Lung Cancer |
|
