|
| Status |
Public on Dec 25, 2012 |
| Title |
CT_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Heart sample3
|
| Organism |
Mus musculus |
| Characteristics |
agent: placebo
strain: C57BL/6
gender: Male
age: 6months
|
| Biomaterial provider |
Charles River
|
| Treatment protocol |
High Fat diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extract Protocol Extraction protocol Total RNA (TRNA) was isolated from blood samples utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000
|
| Description |
RNA was extracted from left ventricle of heart
|
| Data processing |
affymetrix-algorithm-name = ExpressionStat. affymetrix-algorithm-version = 5.0. program-name = Expression Console. Signals were scaled to 150.
|
| |
|
| Submission date |
Nov 19, 2010 |
| Last update date |
Dec 25, 2012 |
| Contact name |
Sara Arab |
| Organization name |
Toronto General Hospital
|
| Department |
Cardiology
|
| Lab |
M. Husain
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
ONT |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE25515 |
Effect of Lira treatment on heart in obese mice |
|
