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Sample GSM628053 Query DataSets for GSM628053
Status Public on Nov 22, 2012
Title KO replicate 1
Sample type RNA
 
Source name bone marrow derived granulocyte monocyte precursors (GMPs) from Gfi1 knock-out mice
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Gfi1 knock-out mice
Growth protocol All mice were housed under SPF conditions and all animal experiments were approved by the institutional animal ethics committee
Extracted molecule total RNA
Extraction protocol total RNA was purified using the Qiagen (Hilden, Germany) Rneasy Micro Kit according to the manufacturers instructions
Label biotin
Label protocol for array hybridization total RNA was amplified and labeled using the following kits from Nugen (San Carlos, California; USA): WT-Ovation Pico Amplification system, WT-Ovation Exon module and FL-Ovation cDNA Biotin Module V2 and the Affymetrix's Hybridization Wash and Stain kit
 
Hybridization protocol hybridization was done following the manufacturers instructions
Scan protocol scanning was done on a Genechip scanner 3000 using the manufacturers instructions
Description KO1
Data processing data was processed using the AltAnalyze software (http://altanalyze.org) using standard RMA analysis settings
 
Submission date Nov 23, 2010
Last update date Nov 22, 2012
Contact name Cyrus Khandanpour
E-mail(s) cyrus.khandanpour@uk-essen.de
Phone +49 20172385212
Organization name University Hospital Essen
Department Hematology
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45147
Country Germany
 
Platform ID GPL10740
Series (1)
GSE25551 Expression data from bone marrow derived granulocyte monocyte precursors (GMPs) from wt -, Gfi1 knock-out -, human-Gfi1-SNP36N knock in and human wt-Gfi1 knock in mice

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
10344625 6.8519
10344626 9.51835
10344627 6.34337
10344628 7.19488
10344629 5.56261
10344630 7.90692
10344631 8.40241
10344632 9.62271
10344634 7.17172
10344635 11.5932
10344636 7.19496
10344639 5.29134
10344640 7.24817
10344641 4.33024
10344642 3.19555
10344644 3.17856
10344645 7.84125
10344646 7.48957
10344647 5.43812
10344648 8.18254

Total number of rows: 105661

Table truncated, full table size 1751 Kbytes.




Supplementary file Size Download File type/resource
GSM628053.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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