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| Status |
Public on Jun 29, 2022 |
| Title |
HHP87Y |
| Sample type |
SRA |
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| Source name |
human hippocampus
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| Organism |
Homo sapiens |
| Characteristics |
age: 87 years old
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| Extracted molecule |
total RNA |
| Extraction protocol |
hippocampal formation samples were homogenized in nuclei lysis buffer, isolated nuclei were loaded on 10X Genomics system.
Chromium Single Cell 3' Reagent Kits v3
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Data processing |
Illumina Casava1.8 software used for basecalling
Reads were aligned to macaca mulatta (release 95) pre-mRNA genome and GRCh38 pre-mRNA genome by Cell Ranger count(v3.0.2)
Alignment results of macaque data and human data were separately aggragated by Cell Ranger aggr(v3.0.2).
Aggragated data of macaque and human were separately read into scanpy.
Assembly: macaca mulatta release 95
Assembly: GRCh38
Supplementary files format and content: The file MHP_year4_11_23_raw.h5ad is formatted by h5ad (created by scanpy) and contains 3 macaque samples; the file HHP_gene_expression _matrix.txt is a tab-separated contains 4 human samples.
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| Submission date |
Jun 29, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Qiang Ma |
| E-mail(s) |
maqiang16@mails.ucas.ac.cn
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| Organization name |
Institute of Biophysics, Chinese Academy of Sciences
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| Street address |
Datun Road, #15
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platform ID |
GPL20301 |
| Series (1) |
| GSE163737 |
Adult hippocampal neurogenesis in aged macaques and humans |
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| Relations |
| BioSample |
SAMN29417128 |
| SRA |
SRX15938509 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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