|
| Status |
Public on Jun 30, 2022 |
| Title |
CK_shoot,biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CK in Rice, shoot
|
| Organism |
Oryza sativa |
| Characteristics |
cultivar: Hitomebore
tissue: shoot-tip
|
| Growth protocol |
Seeds of the two genotypes were thoroughly washed with distilled water and then germinated in the dark in Petri dishes containing distilled water at 28℃. After a 2-day incubation, germinated seeds were transferred to a greenhouse at 26℃ under 16 h/8 h light/dark regime about 48 hours to harvest
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the same seedlings with the Trizol Reagent (Invitrogen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from CK
|
| Data processing |
The data were analyzed with Expression Console using RMA as normalization method.
|
| |
|
| Submission date |
Jun 29, 2022 |
| Last update date |
Jun 30, 2022 |
| Contact name |
Ningning Wang |
| Organization name |
Jilin Agricultural University
|
| Street address |
2888, Xinchen Street
|
| City |
Changchun |
| ZIP/Postal code |
130000 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
|
