|
| Status |
Public on Jan 01, 2011 |
| Title |
tef1:lae1 mutant 1 on lactose_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
T. reesei growing on lactose for 28 hrs
|
| Organism |
Trichoderma reesei |
| Characteristics |
strain: tef1:lae1 overexpression mutant 1
growth protocol: grown on lactose
|
| Treatment protocol |
Mycelia from both stages were freeze-dried and ground in liquid nitrogen using a mortar and pestle. For each of the two experimental conditions, five independent replicates of mycelium were mixed.
|
| Growth protocol |
Trichoderma reesei strains QM9414 (ATCC 26921) and the lae1 deleted strain were grown in 1 l Erlenmeyerflasks flasks on a rotary shaker (250 rpm) at 30 C in the medium described by Seiboth et al. (2004) [Mol Microbiol 51:1015-25] with lactose as a carbon source at a final concentration of 10 g l-1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRIzol® reagent (Invitrogen Life Technologies, Carlsbad, CA, USA), according to the manufacturer's instructions, and then purified using the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). The RNA quality and quantity were determined using a Nanodrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
SAMPLE 5
56936402_532
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Nov 24, 2010 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Christian P. Kubicek |
| E-mail(s) |
ckubicek@mail.zserv.tuwien.ac.at
|
| Phone |
+4315880117250
|
| Fax |
+4315880117299
|
| Organization name |
TU Vienna
|
| Street address |
Getreidemarkt 9
|
| City |
Vienna |
| ZIP/Postal code |
1060 |
| Country |
Austria |
| |
|
| Platform ID |
GPL10642 |
| Series (1) |
| GSE22687 |
Effect of LAE1 gene deletion on gene expression in Trichoderma |
|
