|
| Status |
Public on Jan 11, 2013 |
| Title |
Serum sample from colorectal cancer group (X496) |
| Sample type |
RNA |
| |
|
| Source name |
serum plasma sample
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: colorectal cancer
sample type: serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
20 ml of whole blood from each participant were collected in EDTA tubes. Blood samples were left at 4ºC until plasma separation and plasma was frozen within 6 h of the blood draw. Briefly, samples were centrifuged at 1,600 x g for 10 min at 4 ºC to spin down blood cells, and plasma was transferred into new tubes, followed by further centrifugation at 16,000 x g for 10 minutes at 4ºC to completely remove cellular components. Plasma was then aliquoted and stored at -80°C until use.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared with the Illumina® TotalPrep™ RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Serum sample from colorectal cancer group (X496)
|
| Data processing |
The data were normalised using quantile normalisation with Bioconductor (v2.5) Lumi package.
|
| |
|
| Submission date |
Nov 24, 2010 |
| Last update date |
Jan 11, 2013 |
| Contact name |
María Dolores Giráldez |
| E-mail(s) |
giraldezjimenez@hotmail.com
|
| Phone |
932275418
|
| Fax |
932279387
|
| Organization name |
Hospital Clínic
|
| Department |
Gastroenterology
|
| Lab |
Gastrointestinal & Pancreatic Oncology Group
|
| Street address |
Villarroel 170
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE25609 |
Plasma microRNA profiling by microarray elucidates several candidate biomarkers for colorectal cancer screening |
|
