|
| Status |
Public on Nov 29, 2010 |
| Title |
Arabidopsis_npr1 sni1 ssn2 _triple water_3 |
| Sample type |
RNA |
| |
|
| Source name |
Triple-water-3
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedling
genotype/variation: npr1 sni1 ssn2
treatment: water
time: 16 hours
|
| Treatment protocol |
3-week old npr1 sni1 brca2a mutants were treated with 0.5 mM salicylic acid for 16 hours. Replicates are derived from two (SA treated) or three (water treated) independent biological experiments.
|
| Growth protocol |
Arabidopsis seedlings were grown at 21-24°C, 16-hour day length, 60% relative humidity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the frozen material using Qiagen Rneasy Plant Mini kit
|
| Label |
biotin
|
| Label protocol |
RNA probes were labelled using the Ambion MessageAmp Premier.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix ATH1 GeneChip (Santa Clara, CA, U.S.A).
|
| Scan protocol |
GeneChips were scanned using the Axon Genepix Scanner (Genepix 4000B).
|
| Description |
3-week-old soil grown plants
|
| Data processing |
The GSE23617_SSN2_raw_data.txt data refer to the linear data which has been summarized using a summarization algorithm (RMA16, PLIER 16 and Iterative PLIER 16). All summarization algorithms also do variance stabilization by adding 16.The normalized data are generated after the log transformation and baseline transformation step.
|
| |
|
| Submission date |
Nov 28, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
shui wang |
| E-mail(s) |
swang@sibs.ac.cn
|
| Phone |
9196138175
|
| Organization name |
Duke University
|
| Department |
Biology
|
| Lab |
Xinnian Dong
|
| Street address |
124 Science Dr
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE23617 |
The impact of BRCA2A and SSN2 on plant defense genes |
|
| Relations |
| Reanalyzed by |
GSE118579 |
