|
| Status |
Public on May 01, 2011 |
| Title |
Control 4 b |
| Sample type |
RNA |
| |
|
| Source name |
hiPSC-derived 6-week-old neurons
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Control
gender: Female
|
| Growth protocol |
For neural differentiations, NPCs were dissociated with Accutase and plated in neural differentiation media (DMEM/F12-Glutamax, 1XN2, 1X B27-RA, 20 ng/ml BDNF, 20 ng/ml GDNF (Peprotech), 1 mm dibutyrl-cyclicAMP (Sigma), 200 nm ascorbic acid (Sigma) onto PORN/Laminin-coated plates. 200,000 cells/well of a 6-well plate. hiPSC derived-neurons were differentiated for 6 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in RNA BEE (Tel-test, Inc). RNA was chloroform extracted, pelleted with isopropanol, washed with 70% ethanol and resuspended in water. RNA was treated with RQ1 RNAse-free DNAse (Promega) for 30 minutes at 37C and then the reaction was inactivated by incubation with EGTA Stop buffer at 65C for 10 minutes.
|
| Label |
biotin
|
| Label protocol |
GeneChip WT Sense Target Labeling and Control Reagents (900652) and GeneChip WT cDNA Synthesis and Amplification Kits (900672 and 900673)
|
| |
|
| Hybridization protocol |
As recommended by Affymetrix.
|
| Scan protocol |
GeneChip scanner 3000 7G
|
| Data processing |
Data in the sample data tables are rma background corrected, quantile normalized, log2 transformed and mean probeset summarized. Further processed data on the Series record were performed by Partek Genomics Suite.
|
| |
|
| Submission date |
Nov 29, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Kristen Brennand |
| E-mail(s) |
kristen.brennand@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Psychiatry
|
| Street address |
1425 Madison Ave, 9-20B
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE25673 |
Comparing Control and Schizophrenic hiPSC-derived Neurons |
|
