|
| Status |
Public on Sep 07, 2005 |
| Title |
PA2384-OD 1.3 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
PAO1-23844
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
PA2384 mutant
|
| Growth protocol |
Batch cultures of P. aeruginosa wild type PAO1 and isogenic PA2384 mutant were grown in iron-limited minimal medium C in the computer-controlled bioreactor system. Samples were harvested at different growth stages with OD600 of 0.2, 1.3 and 2.1, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was immediately stabilized with RNAprotect Bacteria Reagent (Qiagen). Samples were stored at -70 oC. Total RNA was extracted with RNeasy mini kit following the protocol recommended by the manufacturer (Qiagen), treated with a DNA-free kit (Ambion) to remove residual chromosomal DNA.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Pseudomonas aeruginosa mutant
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Jul 07, 2005 |
| Last update date |
Jul 07, 2005 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE2885 |
PA2384 and iron starvation in P. aeruginosa |
|
