|
Status |
Public on Sep 07, 2012 |
Title |
Cell line Number 45 rs7222094 genotypeTT stim |
Sample type |
RNA |
|
|
Source name |
B lymphoblastoid cell line stimulated with cytomix for 6 hours
|
Organism |
Homo sapiens |
Characteristics |
cell line number: 45 snp: rs7222094 genotype: TT treatment: stimulated with cytomix for 6 hours cell type: B lymphoblastoid
|
Treatment protocol |
Stimulation with Cytomix for 6 hours, 3 biological replicates.
|
Growth protocol |
Standard tissue culture protocol according to Coriell Respository recommendations
|
Extracted molecule |
total RNA |
Extraction protocol |
Column extraction using QIAgen RNEasy kit.
|
Label |
Biotin
|
Label protocol |
Standard Illumina TotalPrep RNA amplification kit
|
|
|
Hybridization protocol |
Standard Illumina Whole Genome Gene Expression Direct Hybridization Protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
Normalization and fold change were calculated using FlexArray v. 1.4.1 Software package available from Genome Quebec Canada
|
|
|
Submission date |
Nov 30, 2010 |
Last update date |
Sep 07, 2012 |
Contact name |
Simone A Thair |
E-mail(s) |
sthair@stanford.edu
|
Organization name |
Stanford University
|
Department |
Emergency Medicine
|
Street address |
P226 1201 Welch Rd
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE25543 |
Expression Array of Lymphoblastoid Cell lines Under Inflammatory Conditions |
|