|
| Status |
Public on Sep 07, 2012 |
| Title |
Cell line Number 50 rs7222094 genotypeTT stim |
| Sample type |
RNA |
| |
|
| Source name |
B lymphoblastoid cell line stimulated with cytomix for 6 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line number: 50
snp: rs7222094
genotype: TT
treatment: stimulated with cytomix for 6 hours
cell type: B lymphoblastoid
|
| Treatment protocol |
Stimulation with Cytomix for 6 hours, 3 biological replicates.
|
| Growth protocol |
Standard tissue culture protocol according to Coriell Respository recommendations
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Column extraction using QIAgen RNEasy kit.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina TotalPrep RNA amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina Whole Genome Gene Expression Direct Hybridization Protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Normalization and fold change were calculated using FlexArray v. 1.4.1 Software package available from Genome Quebec Canada
|
| |
|
| Submission date |
Nov 30, 2010 |
| Last update date |
Sep 07, 2012 |
| Contact name |
Simone A Thair |
| E-mail(s) |
sthair@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Emergency Medicine
|
| Street address |
P226 1201 Welch Rd
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE25543 |
Expression Array of Lymphoblastoid Cell lines Under Inflammatory Conditions |
|
