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Sample GSM63194 Query DataSets for GSM63194
Status Public on Jul 06, 2006
Title E05_80
Sample type RNA
 
Channel 1
Source name WT (BY4700) after 2h shift from M.Gln to M.Pro
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) after 2h shift from M.Gln to M.Pro
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name gln3∆-gat1∆ (03167b) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics gln3∆-gat1∆ (03167b) on M.Gln
Extracted molecule total RNA
Label Cy5
 
 
Description high laser power and high PMT (Photo Multiplier Tube) gain to detect the faintest spots
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2600
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn R² (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R² (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YBR119W 2240 2030 80 3032 3050 915 683 754 414 98 92 0 7160 7386 1279 2975 3021 677 100 100 0 0.561 0.537 0.574 0.524 1.645 0.479 0.607 52 340 6534 6778 -0.833 2349 4185 2367 4411 158616 384048 5.546 6.448 0 0
2 1 2 1 YBR119W 2440 2040 80 2769 2816 655 696 774 400 100 96 0 6742 6695 1278 3060 3068 695 98 96 0 0.563 0.583 0.551 0.575 1.706 0.471 0.556 52 340 5755 5755 -0.829 2073 3682 2120 3635 146414 348155 5.105 5.219 0 0
3 1 3 1 YBR115C 2640 2030 90 32905 31800 7658 816 877 572 100 100 0 26076 26061 5371 2902 2945 705 100 100 0 1.385 1.338 1.368 1.326 1.215 1.369 0.943 52 420 55263 54143 0.47 32089 23174 30984 23159 1653607 1355184 54.061 32.789 0 0
4 1 4 1 YBR115C 2840 2030 90 28782 29248 9057 867 938 510 100 100 0 24198 24273 6579 2913 2957 681 100 100 0 1.311 1.329 1.339 1.318 1.26 1.357 0.936 52 414 49200 49741 0.391 27915 21285 28381 21360 1520876 1262195 55.51 31.301 0 0
5 1 5 1 YBR099C 3040 2040 120 636 660 356 738 806 454 8 0 0 3658 3888 1175 2897 2954 757 50 33 0 -0.134 -0.079 0.333 0.24 4.774 19.502 0.012 120 684 659 913 null -102 761 -78 991 79162 466549 -0.322 1.234 -50 0
6 1 6 1 YBR099C 3260 2030 70 590 653 379 646 664 333 18 9 0 4812 4750 734 2984 3052 619 90 78 0 -0.031 0.004 0.158 0.149 2.577 868.253 0 32 236 1772 1773 null -56 1828 7 1766 20880 152008 -0.033 2.743 0 0
7 1 7 1 YBR095C 3450 2050 80 2012 2075 591 669 712 392 100 78 0 8419 8563 1748 2854 2906 667 100 98 0 0.241 0.246 0.239 0.238 1.809 0.207 0.544 52 340 6908 7115 -2.051 1343 5565 1406 5709 107918 445263 3.477 8.481 0 0
8 1 8 1 YBR095C 3650 2060 80 1844 1977 709 698 742 397 96 80 0 8571 8565 1367 2918 2937 733 100 100 0 0.203 0.226 0.214 0.212 1.701 0.203 0.457 52 340 6799 6926 -2.302 1146 5653 1279 5647 102802 445389 3.111 7.678 0 0
9 1 9 1 YBR091C 3850 2060 90 1498 1638 554 673 719 371 92 55 0 5104 5197 1003 2754 2842 709 96 86 0 0.351 0.395 0.382 0.373 2.235 0.298 0.326 52 420 3175 3408 -1.51 825 2350 965 2443 85172 270266 2.477 3.322 0 0
10 1 10 1 YBR091C 4050 2070 80 1615 1639 664 609 659 348 84 67 0 5701 5750 1173 2953 2992 661 94 90 0 0.366 0.368 0.33 0.354 2.507 0.291 0.393 52 340 3754 3827 -1.45 1006 2748 1030 2797 85230 299004 2.816 4.172 0 0
11 1 11 1 YBR077C 4250 2060 90 1639 1648 396 582 659 452 88 65 0 7518 7539 1262 2855 2992 869 100 96 0 0.227 0.228 0.223 0.219 1.753 0.218 0.321 52 420 5720 5750 -2.141 1057 4663 1066 4684 85696 392003 2.188 5.232 0 0
12 1 12 1 YBR077C 4460 2060 90 1772 1792 578 613 672 380 90 73 0 7088 7156 1389 2879 2988 837 100 100 0 0.275 0.276 0.295 0.263 1.758 0.192 0.397 52 420 5368 5456 -1.861 1159 4209 1179 4277 93178 372132 2.947 4.98 0 0
13 1 13 1 YBR073W 4660 2060 80 1067 1123 391 599 655 369 59 28 0 4672 4530 915 2781 2862 678 84 65 0 0.247 0.3 0.292 0.3 3.077 0.146 0.087 52 340 2359 2273 -2.015 468 1891 524 1749 58382 235555 1.268 2.46 0 0
14 1 14 1 YBR073W 4850 2050 70 1076 1162 498 620 715 381 59 25 0 5195 5450 1055 2960 3004 728 100 84 0 0.204 0.218 0.271 0.215 2.518 0.105 0.069 32 236 2691 3032 -2.293 456 2235 542 2490 37168 174412 1.173 3.36 0 0
15 1 15 1 YBR069C 5050 2070 80 1122 1238 767 642 648 356 63 26 0 4257 4155 888 2865 2872 635 78 51 0 0.345 0.462 0.403 0.424 3.033 0.262 0.085 52 334 1872 1886 -1.536 480 1392 596 1290 64350 216042 1.657 2.02 0 0
16 1 16 1 YBR069C 5240 2070 120 908 953 490 545 600 354 50 21 0 3360 3534 1120 2844 2893 675 43 28 0 0.703 0.591 0.426 0.46 3.873 0.146 0.054 120 668 879 1098 -0.507 363 516 408 690 114320 424123 0.997 0.95 -50 0
17 1 17 1 YBR053C 5450 2090 80 921 1004 424 540 594 343 53 30 0 5817 5836 1204 2719 2816 733 96 92 0 0.123 0.149 0.132 0.142 2.43 0.105 0.141 52 340 3479 3581 -3.023 381 3098 464 3117 52198 303481 1.195 4.12 0 0
18 1 18 1 YBR053C 5650 2080 80 922 969 451 564 601 327 55 25 0 6491 6237 1122 2966 3004 683 100 96 0 0.102 0.124 0.139 0.127 2.867 0.067 0.067 52 340 3883 3676 -3.3 358 3525 405 3271 50370 324298 1.125 4.734 0 0
19 1 19 1 YBR049C 5860 2080 80 1803 1813 612 524 589 356 94 86 0 7235 7282 1366 2972 3021 628 100 100 0 0.3 0.299 0.29 0.274 1.948 0.256 0.53 52 340 5542 5599 -1.737 1279 4263 1289 4310 94270 378653 3.438 6.785 0 0
20 1 20 1 YBR049C 6060 2090 80 1644 1724 636 558 596 371 86 75 0 7633 7498 1370 2972 3003 721 100 98 0 0.233 0.258 0.252 0.241 2.09 0.22 0.418 52 334 5747 5692 -2.102 1086 4661 1166 4526 89666 389888 3.04 6.234 0 0

Total number of rows: 15488

Table truncated, full table size 3357 Kbytes.




Supplementary data files not provided

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