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Sample GSM63196 Query DataSets for GSM63196
Status Public on Jul 06, 2006
Title E02_100
Sample type RNA
 
Channel 1
Source name WT (BY4700) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Gln
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT (BY4700) on M.Gln + rapamycin (20 min)
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Gln + rapamycin (20 min)
Extracted molecule total RNA
Label Cy5
 
 
Description scan at high laser power and high PMT gain to detect the faintest spots
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2602
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn Rē (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn Rē (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YMR122wa_ OL 4060 23990 120 42006 40364 11054 602 699 555 99 99 0 65535 62870 9203 1491 1615 830 100 100 77 0.646 0.648 0.652 0.613 1.376 0.65 0.916 120 748 105448 101141 -0.629 41404 64044 39762 61379 4843692 7544376 71.468 73.801 0 0
2 1 2 1 YMR122wa_ OL 4280 23990 120 46806 44324 12902 591 716 625 99 99 0 65535 62994 8742 1556 1678 891 100 100 76 0.722 0.712 0.725 0.654 1.554 0.719 0.904 120 725 110194 105171 -0.469 46215 63979 43733 61438 5318843 7559274 69.773 68.817 0 0
3 1 3 1 YMR225c_ OL 4500 23980 110 2172 2289 1009 622 767 648 87 65 0 7114 7281 1960 1550 1639 705 100 100 0 0.279 0.291 0.295 0.263 2.133 0.235 0.336 80 630 7114 7398 -1.844 1550 5564 1667 5731 183157 582463 2.349 8.003 0 0
4 1 4 1 YMR225c_ OL 4720 23980 110 2212 2329 1160 638 774 676 80 55 0 8539 8759 1818 1615 1721 742 100 100 0 0.227 0.237 0.205 0.194 2.171 0.193 0.282 80 622 8498 8835 -2.137 1574 6924 1691 7144 186344 700701 2.3 9.485 0 0
5 1 5 1 YAL066w_ OL 4940 23970 120 618 714 622 677 796 593 12 4 0 1613 1705 619 1729 1803 750 10 0 0 0.509 -1.542 0.864 0.718 3.873 0.092 0.001 120 711 -175 13 -0.975 -59 -116 37 -24 85622 204612 -0.138 -0.131 -50 0
6 1 6 1 YAL066w_ OL 5160 23970 120 693 842 593 714 813 588 20 5 0 1554 1696 711 1672 1738 693 15 4 0 0.178 5.333 0.724 0.897 5.029 3.721 0.007 120 696 -139 152 -2.49 -21 -118 128 24 101025 203514 0.049 -0.061 -50 0
7 1 7 1 YCL001wa_ OL 5380 23970 120 685 770 505 689 816 661 15 1 0 2023 2063 832 1649 1741 731 30 11 0 -0.011 0.196 0.667 0.724 4.86 0.041 0 120 711 370 495 null -4 374 81 414 92423 247580 -0.07 0.44 -50 0
8 1 8 1 YCL001wa_ OL 5600 23970 120 669 847 685 654 808 677 20 6 0 1828 1818 681 1710 1831 792 15 4 0 0.127 1.787 0.872 0.854 4.908 0.079 0 120 710 133 301 -2.976 15 118 193 108 101631 218205 0.058 -0.016 -50 0
9 1 9 1 empty 5820 23970 120 594 780 623 699 805 560 23 7 0 1799 1850 719 1670 1794 809 17 5 0 -0.814 0.45 1.007 0.891 3.766 14.973 0.002 120 696 24 261 null -105 129 81 180 93546 222044 -0.045 0.069 -50 0
10 1 10 1 empty 6040 23970 120 687 794 655 673 794 588 17 5 0 1792 1875 731 1663 1772 836 16 4 0 0.109 0.571 0.609 0.657 4.521 0.508 0.05 120 688 143 333 -3.204 14 129 121 212 95308 225030 0 0.123 -50 0
11 1 11 1 empty 6260 23970 120 723 782 543 613 747 607 21 3 0 1854 1831 814 1710 1832 853 16 4 0 0.764 1.397 0.683 0.561 3.722 0.063 0.002 120 710 254 290 -0.389 110 144 169 121 93781 219676 0.058 -0.001 -50 0
12 1 12 1 empty 6480 23970 120 566 697 574 702 774 566 16 3 0 1769 1891 785 1829 1944 864 11 5 0 2.267 -0.081 0.924 0.961 3.455 32.153 0.001 120 696 -196 57 1.181 -136 -60 -5 62 83697 226909 -0.136 -0.061 -50 0
13 1 13 1 empty 6700 23970 120 508 707 654 703 800 604 18 6 0 1751 1836 728 1821 1971 880 12 2 0 2.786 0.267 1.068 1.172 4.198 0.045 0.001 120 700 -265 19 1.478 -195 -70 4 15 84871 220262 -0.154 -0.153 -50 0
14 1 14 1 empty 6910 23970 100 812 1068 754 1057 1595 1810 3 0 0 1915 2075 766 2075 2816 3306 0 0 0 1.531 100000 1.014 0.884 3.519 0.598 0.284 80 533 -405 11 0.615 -245 -160 11 0 85401 165982 -0.291 -0.224 -50 0
15 1 15 1 empty 7150 23970 120 997 1147 788 1000 1479 1663 6 0 0 2261 2338 858 2152 2750 2907 0 0 0 -0.028 0.79 1.106 0.955 3.766 0.789 0.324 120 728 106 333 null -3 109 147 186 137650 280537 -0.2 -0.142 -50 0
16 1 16 1 empty 7370 23960 120 660 841 626 761 858 599 18 8 0 2134 2334 1027 2015 2141 883 21 10 0 -0.849 0.251 0.725 0.743 3.779 0.029 0 120 682 18 399 null -101 119 80 319 100975 280073 -0.028 0.219 -50 0
17 1 17 1 Mito_SC.11667.11957.ORF8_OL 7590 23960 120 720 848 656 763 889 653 17 4 0 2077 2252 809 2207 2338 924 15 3 0 0.331 1.889 0.637 0.645 4.324 0.03 0 120 682 -173 130 -1.596 -43 -130 85 45 101725 270255 -0.063 -0.093 -50 0
18 1 18 1 Mito_SC.11667.11957.ORF8_OL 7810 23960 120 614 708 574 724 828 615 12 5 0 2000 2211 1036 2357 2494 895 14 4 0 0.308 0.11 0.578 0.54 3.679 0.036 0.001 120 694 -467 -162 -1.698 -110 -357 -16 -146 84925 265267 -0.195 -0.316 -50 0
19 1 19 1 YKL 182W.5310.5380_OL 8040 23970 130 32944 31008 9892 789 886 637 98 98 0 65535 62667 10089 2397 2504 962 99 99 78 0.509 0.501 0.509 0.449 1.623 0.498 0.899 120 784 95293 90489 -0.973 32155 63138 30219 60270 3720971 7520007 47.287 62.54 0 0
20 1 20 1 YKL 182W.5310.5380_OL 8260 23970 130 34543 33421 11269 898 959 710 99 98 0 65535 63283 7833 2484 2859 3633 100 100 78 0.534 0.535 0.535 0.487 1.617 0.529 0.848 120 822 96696 93322 -0.906 33645 63051 32523 60799 4010489 7593946 45.721 16.632 0 0

Total number of rows: 12800

Table truncated, full table size 2923 Kbytes.




Supplementary data files not provided

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