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Sample GSM63197 Query DataSets for GSM63197
Status Public on Jul 06, 2006
Title E07_50
Sample type RNA
 
Channel 1
Source name gln3∆-gat1∆ (03167b) M.Gln + rapamycin (20 min)
Organism Saccharomyces cerevisiae
Characteristics gln3∆-gat1∆ (03167b) M.Gln + rapamycin (20 min)
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT (BY4700) on M.Gln + rapamycin (20 min)
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Gln + rapamycin (20 min)
Extracted molecule total RNA
Label Cy5
 
 
Description scan at low laser power and a PMT gain avoiding saturation
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2602
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn R² (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R² (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YMR122wa_ OL 4060 23980 110 4906 4894 703 66 102 260 100 100 0 19677 19634 2178 148 230 631 100 100 0 0.248 0.248 0.247 0.246 1.135 0.25 0.954 80 642 24369 24314 -2.013 4840 19529 4828 19486 391508 1570683 18.431 30.751 0 0
2 1 2 1 YMR122wa_ OL 4270 23980 110 6115 5972 993 62 79 144 100 100 0 23205 22759 3556 150 238 630 100 100 0 0.263 0.261 0.265 0.26 1.133 0.259 0.964 80 642 29108 28519 -1.929 6053 23055 5910 22609 477799 1820709 40.924 35.748 0 0
3 1 3 1 YMR225c_ OL 4500 23980 110 239 250 98 62 68 46 96 85 0 716 731 192 166 188 195 100 81 0 0.322 0.333 0.315 0.287 2.105 0.326 0.562 80 637 727 753 -1.636 177 550 188 565 19979 58467 3.957 2.785 0 0
4 1 4 1 YMR225c_ OL 4710 23980 110 220 228 84 47 57 41 98 86 0 654 658 149 159 164 66 100 100 0 0.349 0.363 0.366 0.336 1.757 0.33 0.54 80 622 668 680 -1.517 173 495 181 499 18240 52676 4.171 7.485 0 0
5 1 5 1 YAL066w_ OL 4930 23970 120 66 77 55 52 63 46 25 13 0 167 182 93 150 184 305 0 0 0 0.824 0.781 0.889 0.88 3.684 0.084 0.515 120 711 31 57 -0.28 14 17 25 32 9231 21797 0.304 -0.007 -50 0
6 1 6 1 YAL066w_ OL 5150 23970 120 73 79 48 57 65 45 26 10 0 163 160 68 163 194 299 0 0 0 100000 -7.333 0.766 0.744 3.343 12479.434 0 120 696 16 19 16.61 16 0 22 -3 9446 19143 0.311 -0.114 -50 0
7 1 7 1 YCL001wa_ OL 5370 23970 120 72 82 60 54 62 43 31 14 0 171 179 74 159 167 64 25 8 0 1.5 1.4 0.689 0.711 3.583 0.214 0.013 120 710 30 48 0.585 18 12 28 20 9863 21421 0.465 0.188 -50 0
8 1 8 1 YCL001wa_ OL 5590 23970 120 77 85 49 56 62 43 33 12 0 153 167 70 153 162 66 22 7 0 100000 2.071 0.539 0.589 5.737 0.157 0.017 120 720 21 43 16.61 21 0 29 14 10150 20007 0.535 0.076 -50 0
9 1 9 1 empty 5810 23970 120 61 69 42 52 59 42 25 5 0 150 160 71 157 161 58 20 9 0 -1.286 5.667 0.421 0.421 4.683 0.067 0.003 120 710 2 20 null 9 -7 17 3 8313 19204 0.238 -0.017 -50 0
10 1 10 1 empty 6030 23970 120 51 57 37 51 59 40 14 3 0 150 159 65 153 158 60 21 3 0 0 1 0.504 0.437 3.567 0.006 0 120 710 -3 12 null 0 -3 6 6 6883 19052 -0.05 0.017 -50 0
11 1 11 1 empty 6250 23970 120 58 68 49 52 59 40 23 7 0 139 147 62 159 163 63 11 2 0 -0.3 -1.333 0.613 0.726 3.444 0.038 0.001 120 696 -14 4 null 6 -20 16 -12 8131 17694 0.225 -0.254 -50 0
12 1 12 1 empty 6470 23970 120 43 47 30 54 59 39 10 0 0 162 166 64 165 170 65 15 3 0 3.667 -7 0.457 0.536 3.164 0.032 0.002 120 696 -14 -6 1.874 -11 -3 -7 1 5656 19956 -0.308 -0.062 -50 0
13 1 13 1 empty 6690 23970 120 40 48 35 57 62 39 6 2 0 174 175 66 157 162 63 23 5 0 -1 -0.5 0.563 0.56 4.478 23.975 0.002 120 696 0 9 null -17 17 -9 18 5702 21006 -0.359 0.206 -50 0
14 1 14 1 empty 6910 23970 120 55 58 35 58 64 41 12 1 0 158 173 62 159 166 66 18 5 0 3 0 0.839 0.736 4.864 0.065 0.006 120 696 -4 14 1.585 -3 -1 0 14 6920 20710 -0.146 0.106 -50 0
15 1 15 1 empty 7130 23960 120 50 62 41 55 63 42 16 6 0 164 170 57 166 172 67 12 1 0 2.5 1.75 0.577 0.532 3.429 14.536 0.002 120 682 -7 11 1.322 -5 -2 7 4 7413 20423 -0.024 -0.03 -50 0
16 1 16 1 empty 7350 23960 120 53 60 40 53 62 43 17 5 0 179 186 69 168 174 65 26 6 0 0 0.389 0.538 0.541 3.592 15.162 0.006 120 682 11 25 null 0 11 7 18 7220 22279 -0.047 0.185 -50 0
17 1 17 1 Mito_SC.11667.11957.ORF8_OL 7570 23960 120 61 69 57 55 64 45 22 5 0 160 164 71 178 184 72 10 2 0 -0.333 -1 0.645 0.597 3.642 0.052 0.002 120 709 -12 0 null 6 -18 14 -14 8283 19655 0.111 -0.278 -50 0
18 1 18 1 Mito_SC.11667.11957.ORF8_OL 7800 23960 120 53 63 47 56 66 45 21 7 0 146 152 67 180 187 69 9 0 0 0.088 -0.25 0.735 0.819 4.22 0.022 0 120 742 -37 -21 -3.503 -3 -34 7 -28 7588 18193 -0.067 -0.507 -50 0
19 1 19 1 YKL 182W.5310.5380_OL 8020 23960 110 2856 2568 843 62 75 80 100 98 0 9276 8890 1965 192 240 371 100 100 0 0.308 0.288 0.296 0.264 1.535 0.291 0.911 80 614 11878 11204 -1.701 2794 9084 2506 8698 205462 711224 31.163 23.315 0 0
20 1 20 1 YKL 182W.5310.5380_OL 8250 23970 120 3036 2717 1217 59 68 66 95 92 0 9551 8441 3355 198 227 300 99 96 0 0.318 0.322 0.314 0.291 1.553 0.323 0.949 120 745 12330 10901 -1.652 2977 9353 2658 8243 326075 1012860 40.136 27.38 0 0

Total number of rows: 12800

Table truncated, full table size 2625 Kbytes.




Supplementary data files not provided

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