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Sample GSM63199 Query DataSets for GSM63199
Status Public on Jul 06, 2006
Title E09_30
Sample type RNA
 
Channel 1
Source name gln3∆-gat1∆ (03167b) after 2h shift from M.Gln to M.Pro
Organism Saccharomyces cerevisiae
Characteristics gln3∆-gat1∆ (03167b) after 2h shift from M.Gln to M.Pro
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT (BY4700) after 2h shift from M.Gln to M.Pro
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) after 2h shift from M.Gln to M.Pro
Extracted molecule total RNA
Label Cy5
 
 
Description scan at low laser power and a PMT gain avoiding saturation
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2602
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn R² (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R² (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YMR122wa_ OL 4010 24160 120 1218 1105 359 21 22 9 100 100 0 4587 4271 1175 39 41 14 100 100 0 0.263 0.256 0.259 0.243 1.324 0.258 0.96 120 744 5745 5316 -1.926 1197 4548 1084 4232 132649 512508 120.333 302.143 0 0
2 1 2 1 YMR122wa_ OL 4220 24160 120 1338 1322 383 21 23 10 100 100 0 5541 5167 1474 40 41 15 100 100 0 0.239 0.254 0.249 0.252 1.238 0.248 0.952 120 720 6818 6428 -2.062 1317 5501 1301 5127 158689 620022 129.9 341.733 0 0
3 1 3 1 YMR225c_ OL 4440 24160 110 96 93 25 21 22 10 100 98 0 161 166 33 41 43 17 100 100 0 0.625 0.576 0.602 0.547 1.599 0.502 0.674 80 630 195 197 -0.678 75 120 72 125 7403 13293 7.1 7.235 0 0
4 1 4 1 YMR225c_ OL 4660 24150 110 88 87 24 19 22 10 100 98 0 197 202 44 41 44 17 100 100 0 0.442 0.422 0.397 0.41 1.591 0.378 0.659 80 630 225 229 -1.177 69 156 68 161 6967 16158 6.5 9.294 0 0
5 1 5 1 YAL066w_ OL 4880 24150 120 21 22 10 20 22 11 11 5 0 41 40 14 39 42 16 14 1 0 0.5 2 0.714 0.779 2.871 0.052 0.002 120 710 3 3 -1 1 2 2 1 2626 4824 0 -0.125 -50 0
6 1 6 1 YAL066w_ OL 5100 24150 120 24 25 10 20 23 11 22 5 0 41 41 16 39 41 14 23 3 0 2 2.5 0.75 0.761 3.303 0.093 0.003 120 696 6 7 1 4 2 5 2 2984 4965 0.182 0 -50 0
7 1 7 1 YCL001wa_ OL 5320 24150 120 22 24 11 20 22 11 20 5 0 45 46 20 41 43 16 24 6 0 0.5 0.8 0.6 0.564 3.732 0.009 0 120 710 6 9 -1 2 4 4 5 2903 5526 0.182 0.188 -50 0
8 1 8 1 YCL001wa_ OL 5540 24150 120 25 26 11 21 23 10 30 9 0 50 51 17 39 40 15 38 12 0 0.364 0.417 0.591 0.598 4.135 135.428 0 120 696 15 17 -1.459 4 11 5 12 3112 6105 0.3 0.733 -50 0
9 1 9 1 empty 5760 24150 120 23 24 10 20 22 10 23 6 0 39 40 13 39 41 15 12 2 0 100000 4 0.944 0.851 3.588 9.435 0.008 120 711 3 5 16.61 3 0 4 1 2897 4823 0.2 -0.067 -50 0
10 1 10 1 empty 5980 24140 120 19 21 9 20 23 11 17 0 0 40 44 31 41 43 14 12 4 0 1 0.333 0.667 0.678 3.338 0.116 0.041 120 707 -2 4 0 -1 -1 1 3 2534 5261 -0.182 0.071 -50 0
11 1 11 1 empty 6200 24140 120 19 21 8 20 23 11 13 0 0 37 40 14 41 43 15 11 0 0 0.25 -1 0.536 0.637 2.958 0.015 0 120 708 -5 0 -2 -1 -4 1 -1 2493 4782 -0.182 -0.2 -50 0
12 1 12 1 empty 6420 24140 120 20 23 10 20 22 10 17 6 0 39 40 16 41 43 15 15 3 0 0 -3 0.5 0.54 4.039 44.514 0 120 708 -2 2 null 0 -2 3 -1 2702 4826 0.1 -0.2 -50 0
13 1 13 1 empty 6640 24140 120 18 20 8 20 22 10 13 0 0 45 47 17 40 42 15 30 10 0 -0.4 0 0.592 0.522 3.192 0.018 0 120 696 3 7 null -2 5 0 7 2352 5614 -0.2 0.333 -50 0
14 1 14 1 empty 6860 24140 120 21 23 9 22 23 10 15 3 0 45 47 16 40 43 16 24 8 0 -0.2 0.143 0.435 0.589 3.423 0.048 0.002 120 696 4 8 null -1 5 1 7 2755 5602 0 0.25 -50 0
15 1 15 1 empty 7080 24140 120 21 23 11 21 22 9 20 10 0 42 45 17 41 43 16 23 5 0 0 0.5 0.692 0.694 3.698 0.027 0.001 120 696 1 6 null 0 1 2 4 2772 5409 0.111 0.125 -50 0
16 1 16 1 empty 7300 24140 120 21 21 8 19 21 9 19 2 0 40 42 14 41 44 17 10 2 0 -2 2 0.5 0.564 2.966 18.27 0.005 120 696 1 3 null 2 -1 2 1 2550 4981 0 -0.118 -50 0
17 1 17 1 Mito_SC.11667.11957.ORF8_OL 7520 24140 120 22 23 10 20 22 10 22 6 0 39 42 16 42 44 15 13 5 0 -0.667 100000 0.5 0.585 3.204 17.168 0.001 120 725 -1 3 null 2 -3 3 0 2813 5011 0.1 -0.133 -50 0
18 1 18 1 Mito_SC.11667.11957.ORF8_OL 7750 24140 120 20 23 10 22 23 10 15 5 0 39 40 14 43 45 15 10 2 0 0.5 -0.333 0.577 0.55 3.449 0.046 0.002 120 720 -6 -2 -1 -2 -4 1 -3 2736 4775 0 -0.333 -50 0
19 1 19 1 YKL 182W.5310.5380_OL 7980 24150 120 113 119 43 21 23 11 98 97 0 1083 1022 233 45 47 23 100 100 0 0.089 0.1 0.095 0.091 1.657 0.097 0.787 120 720 1130 1075 -3.496 92 1038 98 977 14297 122665 8.727 42.391 0 0
20 1 20 1 YKL 182W.5310.5380_OL 8200 24140 110 139 145 44 21 23 10 100 100 0 1237 1237 166 45 49 28 100 100 0 0.099 0.104 0.097 0.098 1.486 0.1 0.794 80 618 1310 1316 -3.337 118 1192 124 1192 11600 98980 12.2 42.429 0 0

Total number of rows: 12800

Table truncated, full table size 2453 Kbytes.




Supplementary data files not provided
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