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Sample GSM63201 Query DataSets for GSM63201
Status Public on Jul 06, 2006
Title E04_40
Sample type RNA
 
Channel 1
Source name WT (BY4700) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Gln
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT (BY4700) on M.Pro
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Pro
Extracted molecule total RNA
Label Cy5
 
 
Description scan at low laser power and a PMT gain avoiding saturation
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2603
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn Rē (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn Rē (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YJL214w 1990 1250 120 46 50 26 38 43 26 25 7 0 275 278 84 179 183 57 66 39 0 0.083 0.121 0.203 0.248 3.863 0.062 0.023 120 638 104 111 -3.585 8 96 12 99 6042 33406 0.269 1.667 -50 0
2 1 2 1 YJL214w 2230 1280 60 62 70 36 37 40 23 53 28 0 336 343 70 202 207 62 87 56 0 0.187 0.234 0.182 0.223 3.142 0.087 0.048 32 176 159 174 -2.422 25 134 33 141 2240 10971 1.304 2.194 0 0
3 1 3 1 aceF 2420 1260 130 110 114 55 38 44 37 75 46 0 951 935 202 191 195 50 100 99 0 0.095 0.102 0.106 0.094 1.877 0.098 0.47 120 662 832 820 -3.4 72 760 76 744 13724 112204 1.892 14.8 0 0
4 1 4 1 aceF 2620 1260 130 86 97 50 38 41 24 71 49 0 868 913 222 210 213 49 100 100 0 0.073 0.084 0.083 0.079 2.035 0.085 0.427 120 648 706 762 -3.777 48 658 59 703 11596 109514 2.333 14.286 0 0
5 1 5 1 kdtA 2840 1260 130 82 84 35 38 42 24 69 47 0 921 902 181 213 214 53 100 100 0 0.062 0.067 0.072 0.064 2.034 0.055 0.271 120 684 752 735 -4.008 44 708 46 689 10120 108259 1.75 12.981 0 0
6 1 6 1 kdtA 3050 1260 120 83 90 44 42 46 27 64 37 0 800 817 205 218 224 57 100 100 0 0.07 0.08 0.09 0.081 2.143 0.069 0.271 120 618 623 647 -3.827 41 582 48 599 10830 98025 1.63 10.404 0 0
7 1 7 1 tufA 3260 1260 120 82 87 42 46 50 28 56 26 0 806 850 221 232 238 60 100 100 0 0.063 0.066 0.075 0.071 2.086 0.055 0.193 120 636 610 659 -3.995 36 574 41 618 10430 101943 1.321 10.2 0 0
8 1 8 1 tufA 3470 1260 110 76 78 35 48 51 28 46 18 0 709 716 120 271 275 63 100 100 0 0.064 0.067 0.081 0.081 2.123 0.051 0.091 80 560 466 475 -3.967 28 438 30 445 6266 57281 0.964 7 0 0
9 1 9 1 buffer 3670 1260 120 40 45 26 42 47 27 18 3 0 259 260 60 273 276 59 14 1 0 0.143 -0.231 0.299 0.398 4.28 30.645 0.005 120 592 -16 -10 -2.807 -2 -14 3 -13 5440 31185 -0.074 -0.271 -50 0
10 1 10 1 buffer 3880 1260 120 42 46 23 40 45 25 17 6 0 276 286 61 274 277 60 20 5 0 1 0.5 0.333 0.353 5.076 27.852 0.005 120 587 4 18 0 2 2 6 12 5480 34308 0.04 0.15 -50 0
11 1 11 1 ADN12ngml 4090 1270 130 98 97 41 44 49 27 70 48 0 765 782 199 283 286 61 99 97 0 0.112 0.106 0.113 0.101 2.275 0.086 0.295 120 694 536 552 -3.158 54 482 53 499 11658 93841 1.778 8.131 0 0
12 1 12 1 ADN12ngml 4300 1260 130 85 85 41 50 54 29 54 22 0 736 757 191 333 339 69 100 94 0 0.087 0.083 0.117 0.105 2.402 0.067 0.147 120 696 438 459 -3.525 35 403 35 424 10234 90864 1.069 6.058 0 0
13 1 13 1 ADN25ngml 4520 1270 120 84 92 42 46 49 28 58 33 0 1189 1178 236 316 322 81 100 100 0 0.044 0.053 0.061 0.054 2.115 0.05 0.274 120 650 911 908 -4.522 38 873 46 862 11062 141353 1.536 10.568 0 0
14 1 14 1 ADN25ngml 4740 1270 130 84 85 38 38 43 26 63 43 0 1205 1232 271 260 265 61 100 100 0 0.049 0.048 0.054 0.05 2.019 0.042 0.276 120 620 991 1019 -4.361 46 945 47 972 10154 147818 1.615 15.852 0 0
15 1 15 1 ADN50ngml 4930 1270 140 101 103 48 40 44 27 79 54 0 1515 1529 367 249 253 59 99 99 0 0.048 0.049 0.053 0.051 1.924 0.045 0.395 156 662 1327 1343 -4.375 61 1266 63 1280 16118 238567 2.185 21.627 0 0
16 1 16 1 ADN50ngml 5140 1270 150 108 113 49 40 47 37 74 43 0 1814 1775 449 247 257 122 100 99 0 0.043 0.048 0.048 0.045 1.825 0.042 0.43 156 739 1635 1601 -4.526 68 1567 73 1528 17602 276937 1.784 12.443 0 0
17 1 17 1 ADN100ngml 5350 1270 120 152 156 58 42 49 38 89 75 0 2759 2743 787 269 278 131 100 100 0 0.044 0.046 0.045 0.043 1.684 0.044 0.68 120 608 2600 2588 -4.501 110 2490 114 2474 18660 329134 2.816 18.817 0 0
18 1 18 1 ADN100ngml 5570 1270 130 105 116 54 44 50 27 79 57 0 2273 2237 471 310 316 67 100 100 0 0.031 0.037 0.036 0.037 1.745 0.034 0.374 120 688 2024 1999 -5.008 61 1963 72 1927 13936 268430 2.444 28.672 0 0
19 1 19 1 ADN200ngml 5780 1270 130 87 92 36 44 49 28 65 35 0 1712 1689 407 324 335 74 100 100 0 0.031 0.035 0.037 0.038 1.833 0.028 0.24 120 657 1431 1413 -5.013 43 1388 48 1365 11018 202664 1.536 18.297 0 0
20 1 20 1 ADN200ngml 5990 1280 140 110 112 45 40 45 24 84 69 0 2391 2353 466 311 319 73 100 98 0 0.034 0.035 0.038 0.036 1.709 0.033 0.495 156 709 2150 2114 -4.893 70 2080 72 2042 17472 367024 2.792 27.863 0 0

Total number of rows: 12800

Table truncated, full table size 2547 Kbytes.




Supplementary data files not provided
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