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Sample GSM63204 Query DataSets for GSM63204
Status Public on Jul 06, 2006
Title E06_80
Sample type RNA
 
Channel 1
Source name WT (BY4700) on M.Gln
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Gln
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT (BY4700) after 2h shift from M.Gln to M.Pro
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) after 2h shift from M.Gln to M.Pro
Extracted molecule total RNA
Label Cy5
 
 
Description scan at high laser power and high PMT gain to detect the faintest spots
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2603
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn Rē (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn Rē (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YJL214w 1960 1290 100 929 992 471 448 503 305 63 42 0 4375 4566 1465 2245 2291 638 92 76 0 0.226 0.234 0.238 0.223 2.684 0.134 0.169 80 528 2611 2865 -2.147 481 2130 544 2321 79332 365249 1.603 3.566 0 0
2 1 2 1 YJL214w 2180 1290 100 932 977 567 451 485 289 62 33 0 4177 4206 1124 2163 2234 677 86 72 0 0.239 0.257 0.273 0.255 3.348 0.131 0.094 80 522 2495 2569 -2.066 481 2014 526 2043 78128 336478 1.702 2.913 0 0
3 1 3 1 aceF 2390 1300 110 1784 1941 734 416 458 299 97 90 0 7713 7666 1526 2237 2293 703 100 100 0 0.25 0.281 0.263 0.255 1.838 0.242 0.564 80 575 6844 6954 -2.001 1368 5476 1525 5429 155288 613252 4.96 7.643 0 0
4 1 4 1 aceF 2600 1290 120 2537 2543 832 420 464 299 98 95 0 9969 9836 2547 2294 2369 674 100 98 0 0.276 0.281 0.291 0.272 1.819 0.245 0.679 120 618 9792 9665 -1.858 2117 7675 2123 7542 305176 1180374 6.953 11.079 0 0
5 1 5 1 kdtA 2810 1290 120 1759 1824 624 427 466 301 96 92 0 8770 8943 1973 2315 2366 667 100 99 0 0.206 0.211 0.216 0.2 1.797 0.184 0.564 120 618 7787 8025 -2.277 1332 6455 1397 6628 218848 1073143 4.512 9.861 0 0
6 1 6 1 kdtA 3020 1290 130 1807 1887 683 484 527 323 96 86 0 9146 8942 1829 2246 2289 649 100 100 0 0.192 0.21 0.199 0.19 1.836 0.193 0.59 120 670 8223 8099 -2.383 1323 6900 1403 6696 226494 1073098 4.211 10.251 0 0
7 1 7 1 tufA 3230 1290 120 1843 1905 693 473 508 308 96 88 0 9621 9658 2346 2219 2273 629 100 100 0 0.185 0.192 0.189 0.176 1.845 0.172 0.564 120 618 8772 8871 -2.434 1370 7402 1432 7439 228618 1158910 4.536 11.741 0 0
8 1 8 1 tufA 3440 1290 130 1664 1720 650 441 480 295 95 86 0 9438 9530 2222 2262 2308 632 100 100 0 0.17 0.176 0.176 0.161 1.839 0.157 0.534 120 654 8399 8547 -2.553 1223 7176 1279 7268 206414 1143585 4.203 11.427 0 0
9 1 9 1 buffer 3640 1290 120 426 478 299 458 514 309 15 5 0 2311 2421 683 2441 2481 691 16 3 0 0.246 -1 0.339 0.374 4.182 0.009 0 120 601 -162 0 -2.022 -32 -130 20 -20 57336 290578 -0.117 -0.087 -50 0
10 1 10 1 buffer 3860 1290 120 446 484 291 479 520 319 14 4 0 2222 2367 658 2531 2622 818 7 0 0 0.107 -0.03 0.325 0.37 4.277 0.023 0.002 120 632 -342 -159 -3.227 -33 -309 5 -164 58126 284039 -0.113 -0.312 -50 0
11 1 11 1 ADN12ngml 4070 1310 110 1231 1276 554 508 568 351 78 52 0 8143 8322 1828 2647 2780 870 100 98 0 0.132 0.135 0.14 0.13 2.36 0.093 0.204 80 597 6219 6443 -2.926 723 5496 768 5675 102080 665728 2.017 6.37 0 0
12 1 12 1 ADN12ngml 4290 1300 120 1152 1196 436 520 572 327 77 44 0 8003 8106 2344 2615 2676 760 99 95 0 0.117 0.123 0.114 0.121 2.106 0.09 0.298 120 671 6020 6167 -3.092 632 5388 676 5491 143536 972687 1.908 7.145 0 0
13 1 13 1 ADN25ngml 4510 1300 130 1384 1430 490 537 591 354 85 62 0 11345 11582 2742 2655 2749 782 100 100 0 0.097 0.1 0.096 0.089 2.033 0.086 0.418 120 664 9537 9820 -3.359 847 8690 893 8927 171560 1389857 2.37 11.295 0 0
14 1 14 1 ADN25ngml 4720 1300 120 1061 1077 465 548 599 356 58 33 0 9244 9479 1867 2847 2865 805 100 100 0 0.08 0.08 0.103 0.085 2.096 0.069 0.224 120 573 6910 7161 -3.64 513 6397 529 6632 129288 1137471 1.343 8.216 0 0
15 1 15 1 ADN50ngml 4910 1300 130 1579 1601 625 568 621 365 85 65 0 15277 15332 3543 3118 3169 837 100 100 0 0.083 0.085 0.088 0.078 1.896 0.078 0.505 120 632 13170 13247 -3.588 1011 12159 1033 12214 192086 1839882 2.685 14.532 0 0
16 1 16 1 ADN50ngml 5130 1300 130 1012 1035 430 568 622 354 59 24 0 10070 10391 2825 3201 3224 879 98 97 0 0.065 0.065 0.075 0.073 2.157 0.054 0.172 120 670 7313 7657 -3.951 444 6869 467 7190 124148 1246929 1.167 8.154 0 0
17 1 17 1 ADN100ngml 5330 1300 110 2116 2204 904 575 639 352 98 93 0 20145 20469 4803 3096 3163 971 100 100 0 0.09 0.094 0.088 0.088 1.659 0.084 0.578 80 568 18590 19002 -3.468 1541 17049 1629 17373 176286 1637496 4.446 17.823 0 0
18 1 18 1 ADN100ngml 5540 1310 120 1910 1978 720 554 605 355 93 86 0 18066 17381 4252 2964 3041 981 100 99 0 0.09 0.099 0.097 0.091 1.662 0.096 0.68 120 641 16458 15841 -3.477 1356 15102 1424 14417 237386 2085775 3.868 14.618 0 0
19 1 19 1 ADN200ngml 5760 1310 120 2549 2633 841 492 593 580 97 91 0 24198 24001 5151 2774 3225 4419 98 97 0 0.096 0.101 0.102 0.097 1.495 0.096 0.756 120 623 23481 23368 -3.381 2057 21424 2141 21227 315928 2880163 3.517 4.702 0 0
20 1 20 1 ADN200ngml 5970 1310 120 3684 3750 1118 472 560 568 99 97 0 30683 30084 4912 2645 3101 4423 100 100 0 0.115 0.119 0.112 0.116 1.429 0.115 0.804 120 619 31250 30717 -3.126 3212 28038 3278 27439 450030 3610041 5.616 6.101 0 0

Total number of rows: 12800

Table truncated, full table size 2852 Kbytes.




Supplementary data files not provided
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