NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM63219 Query DataSets for GSM63219
Status Public on Jul 06, 2006
Title E08_80
Sample type RNA
 
Channel 1
Source name gln3∆-gat1∆ (03167b) on M.Pro
Organism Saccharomyces cerevisiae
Characteristics gln3∆-gat1∆ (03167b) on M.Pro
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT (BY4700) on M.Pro
Organism Saccharomyces cerevisiae
Characteristics WT (BY4700) on M.Pro
Extracted molecule total RNA
Label Cy5
 
 
Description scan at high laser power and high PMT gain to detect the faintest spots
Data processing VALUE = Log Ratio (635 /532), NO normalization
 
Submission date Jul 07, 2005
Last update date Jul 11, 2005
Contact name Bart Scherens
Organization name IRMW-ULB
Department Microbiology
Street address Av. E. Gryson, 1
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL2603
Series (1)
GSE2891 Transcriptional response to nitrogen availability in yeast

Data table header descriptions
ID_REF
Block Block that the spot was printed in
Column Column within the block
Row Row within the block
Name ORF Name / Feature Name
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in micron
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532) Ratio of Medians (635/532)
Ratio of Means (635/532) Ratio of Means (635/532)
Median of Ratios (635/532) Median of Ratios (635/532)
Mean of Ratios (635/532) Mean of Ratios (635/532)
Ratios SD (635/532) Ratios SD (635/532)
Rgn Ratio (635/532) Rgn Ratio (635/532)
Rgn R² (635/532) Rgn R ≤ (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of Medians
Sum of Means Sum of Means
VALUE Log ratio (635/532)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635
SNR 532
Flags User defined flag (default=0)
Normalize

Data table
ID_REF Block Column Row Name X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R² (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 1 1 1 YJL214w 2020 1350 120 1878 2051 1039 359 417 290 92 85 0 9498 9503 1618 3999 4034 877 99 98 0 0.276 0.307 0.291 0.259 2.368 0.258 0.468 120 550 7018 7196 -1.856 1519 5499 1692 5504 246124 1140320 5.634 6.236 0 0
2 1 2 1 YJL214w 2230 1360 110 2291 2271 716 474 621 1051 85 31 0 8869 9669 2676 4354 4464 1099 98 91 0 0.402 0.338 0.343 0.344 2.04 0.372 0.209 80 557 6332 7112 -1.313 1817 4515 1797 5315 181668 773528 1.57 4.736 0 0
3 1 3 1 aceF 2430 1350 90 2336 2448 837 446 503 340 100 98 0 13819 13762 2321 4523 4548 866 100 100 0 0.203 0.217 0.225 0.205 1.522 0.208 0.68 52 412 11186 11241 -2.298 1890 9296 2002 9239 127312 715642 5.721 10.64 0 0
4 1 4 1 aceF 2640 1360 90 3609 3925 1383 382 434 273 100 100 0 15079 15091 2451 4670 4727 894 100 100 0 0.31 0.34 0.331 0.323 1.617 0.314 0.714 52 420 13636 13964 -1.69 3227 10409 3543 10421 204114 784744 12.788 11.593 0 0
5 1 5 1 kdtA 2850 1350 90 4441 4344 1393 444 481 296 100 100 0 13947 14567 2958 4869 4911 963 100 100 0 0.44 0.402 0.402 0.393 1.337 0.381 0.846 52 412 13075 13598 -1.183 3997 9078 3900 9698 225864 757487 13.051 10.027 0 0
6 1 6 1 kdtA 3070 1360 100 3269 3480 1481 468 508 361 98 98 0 13030 13171 2953 4804 4856 941 100 100 0 0.341 0.36 0.325 0.339 1.64 0.337 0.734 80 526 11027 11379 -1.554 2801 8226 3012 8367 278428 1053656 8.233 8.836 0 0
7 1 7 1 tufA 3280 1360 110 2508 2513 772 400 454 315 97 93 0 13698 13652 2227 5011 5072 990 100 100 0 0.243 0.245 0.241 0.233 1.448 0.227 0.742 80 574 10795 10754 -2.043 2108 8687 2113 8641 201020 1092127 6.537 8.667 0 0
8 1 8 1 tufA 3490 1360 100 2846 3035 1253 388 426 282 100 100 0 14573 14230 2806 4731 4855 1033 98 98 0 0.25 0.279 0.267 0.264 1.639 0.256 0.653 80 512 12300 12146 -2.001 2458 9842 2647 9499 242784 1138427 9.252 9.076 0 0
9 1 9 1 buffer 3700 1360 120 334 385 278 368 420 292 15 2 0 3190 3215 829 3330 3461 1051 8 0 0 0.243 -0.148 0.427 0.521 4.561 0.019 0.003 120 612 -174 -98 -2.042 -34 -140 17 -115 46250 385758 -0.12 -0.234 -50 0
10 1 10 1 buffer 3910 1360 120 391 870 2744 345 414 316 22 12 0 2620 3101 2240 2710 2806 826 20 10 0 -0.511 1.343 0.367 0.419 3.727 1.098 0.561 120 610 -44 916 null 46 -90 525 391 104376 372072 1.443 0.357 -50 0
11 1 11 1 ADN12ngml 4120 1370 100 3551 3719 1619 340 381 276 97 96 0 10799 10810 3241 2904 2927 721 100 97 0 0.407 0.427 0.42 0.418 1.417 0.408 0.787 80 518 11106 11285 -1.298 3211 7895 3379 7906 297542 864839 12.094 10.933 0 0
12 1 12 1 ADN12ngml 4340 1360 90 3397 3485 1165 406 443 286 100 100 0 12512 12650 3446 4416 4421 1303 100 98 0 0.369 0.374 0.396 0.385 1.649 0.324 0.733 52 412 11087 11313 -1.437 2991 8096 3079 8234 181230 657808 10.636 6.315 0 0
13 1 13 1 ADN25ngml 4560 1360 110 4991 4999 1683 384 452 310 100 100 0 17476 17153 3530 4758 4865 1126 100 100 0 0.362 0.372 0.355 0.362 1.598 0.346 0.767 80 569 17325 17010 -1.465 4607 12718 4615 12395 399902 1372251 14.668 10.913 0 0
14 1 14 1 ADN25ngml 4760 1370 110 6414 6515 2236 353 421 321 100 98 0 20254 20390 3946 4088 4135 970 100 100 0 0.375 0.378 0.38 0.36 1.432 0.378 0.858 80 560 22227 22464 -1.415 6061 16166 6162 16302 521198 1631166 18.984 16.758 0 0
15 1 15 1 ADN50ngml 4960 1360 110 8058 8193 3185 394 445 309 100 100 0 24772 22781 7369 3956 4018 899 100 98 0 0.368 0.414 0.417 0.425 1.462 0.397 0.876 80 547 28480 26624 -1.442 7664 20816 7799 18825 655428 1822468 25.074 20.871 0 0
16 1 16 1 ADN50ngml 5170 1360 110 7661 7625 3132 384 438 310 100 97 0 21593 21257 6727 3866 3914 889 100 100 0 0.411 0.416 0.423 0.407 1.403 0.404 0.884 80 563 25004 24632 -1.285 7277 17727 7241 17391 609986 1700561 23.184 19.508 0 0
17 1 17 1 ADN100ngml 5390 1370 110 17042 16983 5578 390 446 301 100 100 0 46332 46592 9856 3849 3930 848 100 100 0 0.392 0.388 0.382 0.374 1.346 0.395 0.934 80 572 59135 59336 -1.351 16652 42483 16593 42743 1358616 3727324 54.94 50.309 0 0
18 1 18 1 ADN100ngml 5600 1370 110 16836 16441 5498 380 438 351 100 100 0 43737 43437 8792 3795 3825 796 100 100 0 0.412 0.405 0.404 0.389 1.3 0.414 0.935 80 565 56398 55703 -1.279 16456 39942 16061 39642 1315308 3474970 45.593 49.764 0 0
19 1 19 1 ADN200ngml 5810 1380 110 9983 14335 8156 390 432 296 100 100 0 37718 39634 11587 3923 3936 851 100 100 0 0.284 0.39 0.372 0.348 1.422 0.421 0.889 80 571 43388 49656 -1.817 9593 33795 13945 35711 1146769 3170716 46.97 41.948 0 0
20 1 20 1 ADN200ngml 6020 1380 100 15696 15452 5160 380 438 313 100 100 0 47155 46400 9104 3767 3896 1048 100 100 0 0.353 0.354 0.353 0.34 1.325 0.361 0.914 80 500 58704 57705 -1.502 15316 43388 15072 42633 1236126 3711993 47.968 40.557 0 0

Total number of rows: 12800

Table truncated, full table size 2915 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap