|
| Status |
Public on Feb 21, 2012 |
| Title |
Lentiviral construct containing Tdrd7 shRNA rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
21EM15 mouse lens epithelial cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell type: lens epithelial cell line
cell line: 21EM15
genotype/variation: Lentiviral construct containing Tdrd7 shRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. DNAse treatment was performed according to Qiagen kit instructions. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Ambion) for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalized using rank invariant normalization using Illumina's GenomeStudio2010.2 v 1.7.0
|
| |
|
| Submission date |
Dec 02, 2010 |
| Last update date |
Feb 21, 2012 |
| Contact name |
Anton Aboukhalil |
| Organization name |
Massachusetts Institute of Technology
|
| Street address |
77 Avenue Louis Pasteur, 464
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE25774 |
Genome-wide analysis of Tdrd7 knockdown in lens epithelial-derived cell line 21EM15 |
| GSE25812 |
Mutations in the RNA Granule Component TDRD7 Cause Cataract and Glaucoma |
|
