|
Status |
Public on Dec 25, 2012 |
Title |
30 min post infection - donor B CRAM_OHU28K-232_2-10-05 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
L.(V.)panamensis infected human macrophage
|
Organism |
Homo sapiens |
Characteristics |
cell type: monocyte differentiated macrophage infection: L.(V.)panamensis individual: donor B time: 30 min post infection
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA samples were extracted with Trizol from adherent macrophages, and RNA recovery was done with Rneasy Micro Kit (Qiagen).
|
Label |
Cy5
|
Label protocol |
cDNA was ligated to the appropriate Cy5/Cy3 3DNA capture sequence
|
|
|
Channel 2 |
Source name |
human macrophage
|
Organism |
Homo sapiens |
Characteristics |
cell type: monocyte differentiated macrophage infection: control individual: donor B
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA samples were extracted with Trizol from adherent macrophages, and RNA recovery was done with Rneasy Micro Kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
cDNA was ligated to the appropriate Cy5/Cy3 3DNA capture sequence
|
|
|
|
Hybridization protocol |
Labelled cDNA was hybridized to a 70-mer OHU28K Human oligo array, after addition of 1ug of Human Cot-1 DNA to the hybridization mixture in a 22x71 mm AdvaCard. Hybridization was done overnight at 55ÂșC, followed by washings with 2XSSC/0.2%SDS.
|
Scan protocol |
Arrays were scanned with Axon GenePix 4000A laser confocal scanner at 532 and 635 nm.
|
Description |
30 min post infection - donor B
|
Data processing |
Image files were analyzed with GenePix Pro Software and GeneSpring GX 7.2 software was used for gene expression data analysis. Lowess normalization was performed to eliminate dye-related artifacts.
|
|
|
Submission date |
Dec 02, 2010 |
Last update date |
Dec 25, 2012 |
Contact name |
Diane McMahon-Pratt |
Organization name |
Yale School of Public Health
|
Department |
Epidemiology of Microbial Diseases
|
Lab |
LEPH 811
|
Street address |
60 College St
|
City |
New Haven |
State/province |
Ct |
ZIP/Postal code |
06511 |
Country |
USA |
|
|
Platform ID |
GPL4693 |
Series (1) |
GSE25819 |
Human Macrophage Response to Leishmania (Viannia) panamensis Infection: Evidence for an Early Inflammatory Response |
|