|
| Status |
Public on Jul 01, 2011 |
| Title |
Human ES cells replicate 1 - membrane polysome associated polysome fractionation - run2 |
| Sample type |
SRA |
| |
|
| Source name |
human ES cells
|
| Organism |
Homo sapiens |
| Characteristics |
strain: HES2
|
| Treatment protocol |
cells were treated with cycloheximide for 10 minutes prior to harvesting (Kolle et al., 2009)
|
| Growth protocol |
HES2 cells were cultured as per Kolle et al., stem cells 2009
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Solid Whole transcriptome analysis Kit - RNA fragmented to ~100bp, RNA-DNA hybrid adaptors ligated, cDNA made, PCR amplified and gel purified
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
AB SOLiD System 2.0 |
| |
|
| Description |
membrane associated RNA from human ES cells
|
| Data processing |
Raw data processed with Bioscope 1.2
|
| |
|
| Submission date |
Dec 03, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Sean Grimmond |
| E-mail(s) |
s.grimmond@imb.uq.edu.au
|
| Organization name |
University of Queensland
|
| Department |
Institute for Molecular Bioscience
|
| Lab |
Expression Genomics Lab
|
| Street address |
Services Rd
|
| City |
St Lucia |
| State/province |
Q |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL9138 |
| Series (1) |
| GSE25842 |
Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human Embryonic Stem cells (part 2) |
|
| Relations |
| SRA |
SRX038750 |
| BioSample |
SAMN00191381 |
