|
Status |
Public on Jul 01, 2011 |
Title |
Human ES cells replicate 2 - mRNA run 1 |
Sample type |
SRA |
|
|
Source name |
human ES cells
|
Organism |
Homo sapiens |
Characteristics |
strain: HES2
|
Treatment protocol |
cells were treated with cycloheximide for 10 minutes prior to harvesting (Kolle et al., 2009)
|
Growth protocol |
HES2 cells were cultured as per Kolle et al., stem cells 2009
|
Extracted molecule |
total RNA |
Extraction protocol |
Solid Whole transcriptome analysis Kit - RNA fragmented to ~100bp, RNA-DNA hybrid adaptors ligated, cDNA made, PCR amplified and gel purified
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
AB SOLiD System 2.0 |
|
|
Description |
mRNA from human ES cells
|
Data processing |
Raw data processed with Bioscope 1.2
|
|
|
Submission date |
Dec 03, 2010 |
Last update date |
May 15, 2019 |
Contact name |
Sean Grimmond |
E-mail(s) |
s.grimmond@imb.uq.edu.au
|
Organization name |
University of Queensland
|
Department |
Institute for Molecular Bioscience
|
Lab |
Expression Genomics Lab
|
Street address |
Services Rd
|
City |
St Lucia |
State/province |
Q |
ZIP/Postal code |
4072 |
Country |
Australia |
|
|
Platform ID |
GPL9138 |
Series (1) |
GSE25842 |
Deep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human Embryonic Stem cells (part 2) |
|
Relations |
SRA |
SRX038759 |
BioSample |
SAMN00191390 |